Tissue profiling

Uys J, Grey A, Wiggins A, Schwacke J, Schey K, Kalivas P (2010) Matrix-assisted laser desorption/ionization tissue profiling of secretoneurin in the nucleus accumbens shell from cocaine-sensitized rats. J Mass Spectrom 45 97-103. doi 10.1002/jms.l697... 

Uhlen M, Ponten F. Antibody-based proteomics for human tissue profiling. Mol Cell Proteomics 2005 4(4) 384-393. Review. 

Keywords Proteomics Genomics Neuroproteomics 2 dimensional electrophoresis Protein profiling Protein fingerprinting Biomarker Systems biology Tissue profiling Bioinformatics... 

Early attempts to develop patterns of specific compounds and proteins were based on tissue homogenates (Bums, 1982 Jimenez et al., 1997 Mitchell et al., 1997). Tissue profiling, or more accurately called tissue imaging, based on direct mass spectrometry measurements is a much younger area of research which started being developed in late 90s when MALDI-TOF-MS measurements were performed on intact tissue sections (Chaurand et al., 1999). 

Caldwell RL, Caprioli RM (2005) Tissue profiling by mass spectrometry A review of methodology and applications. Mol Cell Pro teomics 4 394-401. 

Thibault, D.B. et al., MALDI tissue profiling of integral membrane proteins from ocular tissues, J. Am. Soc. Mass Spectrom., 19(6), 814, 2008. 

Cornett, D.S. et al., A novel histology directed strategy for MALDI-MS tissue profiling that improves throughput and cellular specificity in human breast cancer, Mol. Cell. Proteomics, 5(10), 1975, 2006. 

Nilsson P, Paavilainen L, Larsson K, et al. Towards a human proteome atlas High-throughput generation of mono-specific antibodies for tissue profiling. Proteomics. 2005 5 4327. 

Blasberg, R., C. Patlak, and J. Fenstermacher, Intrathecal chemotherapy brain tissue profiles after ventriculocisternal perfusion. Journal of Pharmacology and Experimental Therapeutics, 1975, 195, 73-83. 

Tissues. Profiles of the distribution of chloroammlneplatlnum(II) complexes as a function of time (for periods up to 7 d) in four specific tissues are shown in Figure 3. The distribution patterns are distinct and presumably reflect the unique chemical behavior expected for discrete substitution-inert complexes, l.e., these complexes appear to retain sufficient identity in vivo to exhibit characteristic distribution patterns. This can be contrasted with the behavior of simple binary metal salts, which exist as labile cations in solution, and whose distribution properties show little or no difference from one salt to another. All compounds are cleared relatively rapidly from the blood (panel A), especially the cationic species, [Pt(NH3)3d] and [Pt(NH3) ] , for which the levels are on the order of 0.1% and 0.02%, respectively after 4 h. The [Pt(NH3)Cl3] ion shows the highest level after 7 d (-v-U). 

Fingerprinting and nontargeted metabonomies benefit from an unbiased view of all eompound elasses. In these approaehes, the endogenous metabolites that might be of interest are not known a priori, and, therefore, sample preparation teehniques that preserve as mueh of the metabolome as possible are essential. There are a number of reports in the literature that deseribe tissue profiling, but those teehniques are generally more speeifie for partieular tissues (i.e., brain, liver, muscle, etc.) and/or expected metabolites of interest. In general, for fatty... 

Zimmerman, T. A., Monroe, E. B., Sweedler, J. V. (2008) Adapting the stretched sample method from tissue profiling to imaging. Pro-teomics, 8, 3809-3815. 

Biomolecules Separation by Ion Mobility in Tissue Profiling Experiments... 

We have used MALDI IM not just for direct tissue profiling, but also for the imaging of lipids in the brain, as MALDI IM is a versatile analytical technique that allows us to map and image the distribution of biomolecules and elucidation of their molecular structure with minimal preparation. ) Figure 12.5 illustrates the imaging of a brain... 

---