Two-Dimensional Electrophoresis of Proteins

P. H. O Eaixell, High resolution two-dimensional electrophoresis of proteins , 7. Biol. Chem. 250 4007 (1975). 

KLOSE, J., KOBALZ, U., Two-dimensional electrophoresis of proteins An updated protocol and implications for a functional analysis of the genome, Electrophoresis, 1995,16,1034-1059. 

P. O Farrell, J. Biol. Chem. 250, 4007-4021 (1975). High Resolution Two-Dimensional Electrophoresis of Proteins. ... 

Gorg, A., and Weiss, W. (1998). High-resolution two-dimensional electrophoresis of proteins using immobilized pH gradients. In Cell Biology A Laboratory Handbook (J. E. Celis, ed.), 2nd ed., Vol. 4, pp. 386-397. Academic Press, San Diego, CA. 

One common method that will not be considered here is two-dimensional electrophoresis of proteins prior to protein identification. This topic has been extensively reviewed elsewhere (2). Furthermore, this chapter will not attempt to review the different instruments available to analyze the samples using mass spectrometry. Some instruments are capable of analyzing proteins directly (for example, MS analysis using a MALDI-based platform) and others analyze peptides (for example, LC MS/MS), derived from endogenous proteins by the action of proteases. The protease employed is very often, but not exclusively, trypsin and the analysis of the peptides allows the parent protein to be identified. 

O Farrel, P H. (1975) High resoluuon two-dimensional electrophoresis of proteins. J. BtoL Chem 250, 4007-4021. 

Ressler.N (1960) Two dimensional electrophoresis of protein-antigens with an anu-bod)K ontaining buffer. Cbn. Chxm. Ada 5, 795-600... 

Figure 11.10. Two-dimensional separation of E. coli protein mixture, using IEF with carrier ampholytes, pH range 3-10, in the first (horizontal) direction, and SDS-PAGE was mn from top to bottom in the second dimension on a 9-14% polyacrylamide gradient running gel cast with 0.1% SDS.12 [Reprinted, with permission, from P. H. O Farrell, The Journal of Biological Chemistry 250 (No. 10 May 25), 1975, 4007 4021. High Resolution Two-Dimensional Electrophoresis of Proteins . Copyright 1975 by the American Society for Biochemistry and Molecular Biology, Inc.]...

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