Proteomics and Tissue Profiling

Early attempts to develop patterns of specific compounds and proteins were based on tissue homogenates (Bums, 1982 Jimenez et al., 1997 Mitchell et al., 1997). Tissue profiling, or more accurately called tissue imaging, based on direct mass spectrometry measurements is a much younger area of research which started being developed in late 90s when MALDI-TOF-MS measurements were performed on intact tissue sections (Chaurand et al., 1999). 

An important issue that arouse during early development of proteomic technology is proper comparison of data generated by different centers, laboratories, even individuals. SELDI-TOF is an example of how difficult it w as to cross-validate data from laboratory to laboratory w hich used the same technology 

How does studying the proteome compai e to studying the genome  

What is the difference between biomarker and risk marker  

Explain in which cases SELDI-TOF technology will be more advantageous than LC-MS/MS and/or MALDI-TOF. 

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