Tips and Tricks

Low Mass Region. All spectra shown in the examples were acquired using the quadmpole ion trap mass spectrometer. As noted previously, this widely used and relatively cheap mass analyzer suffers the low-mass cut-off phenomena. In addition to techniques used in the examples shown above, other mass analyzers applied for tandem mass spectrometers may cover the low mass region of the fragmentation spectmm that can be information rich. 

First of all, the shortest fragments in both b- and y-ion series should be available. Such ions can be used to assign the sequence strings to either N- or C-terminal ion 

Enzymatic Digestion. If the peptide to be sequenced is in a pure form or can be isolated and purified, the more classic methods can be used to support de novo sequencing. However, they are unlikely to provide good results when working with mixtures of peptides. Below, several additional techniques are listed. 

Edman Degradation. This technique requires more material than MS-based sequencing and its sensitivity decreases with the number of amino acids detected. The use of Edman degradation sometimes allows determination of those N-terminal amino acids that were not detected during MS sequencing. 

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There are quite a few Web pages on the World Wide Web that address topics related to SAS. Many are organized in SAS Web rings, so that when you find one you can link to the others. Most of these Web pages are hosted by third-party SAS consultants and solution providers, but they can provide some good SAS tips and tricks. Simply do an Internet search on SAS to find these Web sites. 

Putting all the annoying exceptions into one corral Handling exceptions more easily with tips and tricks... 

The LIC system is adaptable for use with any vector following an alteration of the vector sequence. This chapter describes the creation of an LIC-compatible vector, with tips on how to make any vector LIC-enabled. It also includes a protocol for generating high-quality linearized vector template for the LIC reaction. Lastly, a step-by-step protocol of the LIC reaction is outlined, with useful tips and tricks for optimization and screening. 

General Tips and Tricks of Helium Leak Detection... 

Tips and tricks Guidance for quality unit laboratories Summary of key ingredients of each method Safety issues/precautions Use of compendial methods... 

Cases can seem daunting at first, but with a little practice, they can be a fun and interesting challenge, as well as the first step to a new job. Exhibit 6-2 offers some tips and tricks from an experienced management consultant. 

Math Survival Guide Tips and Tricks for Science Students, 2nd Edition, a paperback summary of basic skills with practice exercises in every chapter. Foundations of Chemistry in the Laboratory, 13th Edition, by Morris Hein, Judith N. Peisen and Robert L. Miner includes 28 experiments for a laboratory program that may accompany the lecture course. Featuring updated information on waste disposal and emphasizing safe laboratory procedures, the lab manual also includes study aids and exercises. 

The perspectives of the DMU look forward to integrate information that originally was not meant for a mock-up and therefore was no requisite for the DMU development such as the aesthetic intentions of the design [34], concepts and inspirations, best practices, tips and tricks that may not be well documented in the PLM strategy. 

Morgan, R. 2006. Tips and Tricks for Recycling Old Computers. SmartBiz. http //www. smartbiz.com/article/articleprint/1525/-l/58 (accessed on 1 December 2013). 

A discussion forum for QSAR researchers is the WWW home page of The QSAR and Modelling Society at http //www. pharma.ethz.ch/qsar Not only names and e-mail addresses of QSAR colleagues but also tips and tricks, information on recent books and software, and links to related topics can be found there. 

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