Time-resolved fluoro-immunoassay

Charbonniere LJ, Hildebrandt N, Ziessel RF, Lohmannsroben HG (2006) Lanthanides to quantum dots resonance energy transfer in time-resolved fluoro-immunoassays and luminescence microscopy. J Am Chem Soc 128 12800-12809... 

The application of the Horrock and Sudnick equation to the terbium complex of ligand 6, reveals that only one water molecule is still coordinated to the complexed cation. Consequently, the lifetime (t = 1.5 sec) and the luminescence quantum yield (< > = 0.2) of this complex are very high and make it attractive for applications as luminescent probes in time-resolved fluoro-immunoassays (TR-FIA). " In order to lurther improve the luminescence properties of these ligands by increasing their molar absorbitivities (e) we have synthesized calix[4]arenes (e.g. 7) with four bipyridines or phenantrolines at the lower rim. A significant increase in the value of e of these derivatives has been... 

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