Identification of Single Molecules

Muller R et al 1996 Time-resolved identification of single molecules in solution with a pulsed semiconductor diode laser Chem. Phys. Lett. 262 716-22... 

Identification of single molecules has relevance for spectroscopic DNA sequencing [379, 475] and drug screening [75]. Molecules can be identified by using the... 

J. Schaffer, A. Volkmer, C. Eggeling, V. Subramaniam, G. Striker, C.A.M. Seidel, Identification of single molecules in aqueous solution by time-resolved fluorescence anisotropy, J. Phys. Chem. A 103, 331-336 (1999)... 

Muller, R, Zander, C, Sauer, M, Deimel, M, Ko, D-S, Sibert, S, arden-Jacob, J, Deltau, G, Marx, NJ, Drexhs e, KH, Wotfirum, J, Time-Resolved identification of single molecules in solution with a pulsed semiconductor diode laser. Chemical Physics Letters 262 (1996) 716-722. 

Knemeyer, JP, Herten, DP, and Sauer, M, Detection and identification of single molecules in living cells using spectrally resolved fluorescence lifetime imaging microscopy. Analytical Chemistry 75 (2003) 2147-2153. 

Becker, W., Hickl, H., Zander, C., et al. 1999. Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single-photon counting (Tcspc). Rev. Sci. Instrum. 70 1835. 

X-ray diffraction or X-ray diffractometry (XRD) is a technique that is useful for the analysis of solid crystalline or semicrystaUine materials. Most organic and inorganic compounds, minerals, metals, and alloys, and many types of polymers form crystals and can be analyzed by XRD. XRD can provide the exact crystal structure of a pure single crystal material. In addition, XRD can provide the quahtative and quantitative identification of the molecules present in pure crystalhne powders or mixtures of crystalline powders. 

Probably the most advanced study with an AFM is the examination of single molecule behavior in its natural environment. AFM was proven as the perfect tool for identification and characterization of single polymer chains [35]. Our future efforts will be in conducting measurements on single polymer chains, compare them with results of other mechanical methods and finally try to correlate both sets of results with the final pol)nner material characteristics. If successful, we will be able to design and predict several novel materials. 

Dahan, M, Deniz, AA, Ha, T, Chemla, DS, Schultz, PG, and Weiss, S, Ratiometric measurement and identification of single diffusing molecules. Chemical Physics 247 (1999) 85-106. 

Part of the RNA molecules is identical for all microorganisins, while other regions are less well conserved. Thus, sequences can be found that are specific for different taxonomic levels, from species, genera, kingdoms, and domains. Pubhc databases contain 16S RNA sequences from many of the descrihed hacterial species. Microbial cells contain 1,000-30,000 copies of 16S RNA molecules, allowing sensitive assays and identification of single cells by fluorescent oligonucleotide hybridization. 

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