Time-lapse cinematography

Target Theory, 124-126, 132 Template chemistry, 170 Thymine dimers, 133-135 Time-lapse cinematography, 249... 

Fig. 10.4. Distribution of generation times of various cell types in culture. The proportion (a) of cells remaining in interphase at various times after division has been recorded for various cells by time lapse cinematography, (a) Rat sarcoma, (b) HeLa S3, (c) mouse fibroblasts (d) L5 cells and (e) HeLa. (Reproduced from Smith and Martin, 1973, with kind permission of the authors and publishers.)...

Temin (1970) and Todaro et al. (1965) showed similar effects for chicken fibroblasts and 3T3 mouse fibroblasts. The low level of serum is important for survival as well as for the subsequent stimulation of DNA synthesis (Cherrington, 1984). A kinetic analysis using time lapse cinematography (Zetterberg and Larsson, 1985) showed that Swiss 3T3 cells were only susceptible to cell cycle arrest in a short period (3-4 h) following mitosis. Even a 1-h exposure to serum-free medium during this time forced the cells into GO from which they required 8 h to return to Gl. The length of the postmitotic sensitive phase was very constant at between 3 and 4 h but considerable intercellular variability existed in the duration of the pre S-phase Gl period consistent with a transition probability event ( 10.4). 

Time-lapse cinematography, originally pioneered by Abercrombie (1953, 1957), who first suggested that locomotory behavior of malignant cells could differ from that of normal cells, has evolved and has been replaced by the more sophisticated time-lapse videomi-croscopy. This technique allowed a more precise description of cell motility, and suggested a correlation between cancer cell motility... 

An even more refined method of study is interference-contrast microscopy [following Nomarski (767)] which converts minute topographical differences into color changes. Replica electron microscopy (77), gold decoration (see Section III,A,1), and scanning electron microscopy are also useful but less practicable. Occasionally it is profitable to follow the course of the reaction, in situ, using time-lapse cinematography (77). 

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