Thermoascus aurantiacus

The a-glucuronidase of T reesei also had an acidic isoelectric point (25). It had a molecular weight of about 70 kDa as estimated by gel chromatography and a pH-optimum at pH 6 with 4-O-methylgucurono-xylobiose as substrate. The very recently characterized a-glucuronidase of the thermophilic fungus Thermoascus aurantiacus (51) was a single polypeptide chain with a MW of... 

Figure 2. Heat inactivation profile of the xylanase in the culture filtrate from Thermoascus aurantiacus. Xylanase enzyme was prepared as described by Tan et al. (74). Xylanase and endocellulase activities were determined after the enzyme was incubated for the specified time at 70°C. Aliquots were removed and assayed at 50°C by the method described by Yu et al. (75). Activities were expressed as a percentage of the control stored at 4°C.

Khandke, K. M., Vithayathil, P. J., andMurthy, S. K., Purification and characterization of an alpha-D-glucuronidase from a thermophilic fungus, Thermoascus aurantiacus. Arch Biochem Biophys 1989, 274 (2), 511-7. 

Fig. 2 Protease production by different thermophilic fungi in SSF using different substrates. Thermoascus aurantiacus, T. lanuginosus, T. lanuginosus TO.03, Aspergillus sp. 13.33, Aspergillus sp. 13.34, Aspergillus sp. 13.35, and Rhizomucor sp. 13.37 3-day fermentation period. Aspergillus flavus 1.2 and R. pusillus 13.36 4-day fermentation period...

The first PMO structure that was resolved is that of Cel61B from Trichoderma reesei By now, five other PMO structures have been studied, i.e. four from ascomycete fungi [Thermoascus aurantiacus, Thielavia terrestris and Neurospora crassa ) and more recently one from the basidiomycete fungus Phanerochaete chrysosporium. Their overall fold will be discussed first, after which a close-up of their active site will be presented. 

The cellulase activities in Cellvibrio fulvus, Chaetomium thermophile, Sporotrichum thermophile and Thermoascus aurantiacus have been examined. The development of cellulolytic enzymes in cultures of Chrysosporium lignorum during growth has been monitored. ... 

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