Terminal fragment length

Liu, W.-T., T. L. Marsh, H. Cheng, and L. J. Forney. 1997. Characterization of microbial diversity by determining terminal fragment length polymorphisms of genes encoding 16S rRNA. Applied and Environmental Microbiology 63 4516-4522. 

Degradation of contaminants may occur with bacteria that have been isolated from pristine environments without established exposure to the contaminants, and exhibit no dependence on substrate concentration. For example, organisms from a previously unexposed forest soil were able to degrade 2,4,6-trichlorophenol at concentrations up to 5000 ppm, and terminal restriction fragment length polymorphism analysis revealed that at concentrations up to 500 ppm, the bacterial community was unaltered (Sanchez et al. 2004). 

FISH, terminal restriction-fragment length polymorphism analysis (rRNA-based molecular techniques) and comparative 16S rDNA analysis... 

T-RFLP Terminal restriction fragment length polymorphism... 

The synthesis of coiled coils of varying lengths and the inclusion of a N-terminal fragment containing a cysteine residue for potential covalent linking of the polypeptide chains to disulfide-bridge coiled coils is shown in Scheme 3. 

Marsh, T. L. 1999. Terminal restriction fragment length polymorphism (T-RFLP) An emerging method for characterizing diversity among homologous populations of amplification products. Current Opinion in Microbiology 2 323-327. 

Osborn, A. M., Moore, E. R. B., and Timmis, K. N. (2000). An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics. Environ. Microbiol. 2,39-50. 

Singh, B. K., Nazaries, L., Munro, S Anderson, I. C and Campbell, C. D. (2006). Use of multiplex terminal restriction fragment length polymorphism for rapid and simultaneous analysis of different components of the soil microbial community. Appl. Environ. Microb. 72, 7278-7285. 

Leuders, T., and Friedrich, M. W. (2003). Evaluation of PGR amplification bias by terminal restriction fragment length polymorphism analysis of smaU-subunit rma and mcra genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts. Appl. Environ. Microbiol. 69, 320-326. 

Moeseneder, M. M., Arrieta, J. M., Muyzer, G., Winter, C., andHemdl, G.J. (1999). Optimization of terminal-restriction fragment length polymorphism analysis for complex marine bacterioplankton communities and comparison with denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 65, 3518-3525. 

Scala, D. J., and Kerkhof, L. J. (2000). Horizontal heterogeneity of denitrifying hacterial communities in marine sediments hy terminal restriction fragment length polymorphism analysis. Appl. Environ. Microbiol. 66, 1980—1986. 

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