Template specificity

DNA polymerases from several different animal cells have been isolated and studied. The three DNA polymerases of animal cells, called a, p, and y, can be distinguished by their molecular weights, template specificity, and sensitivity to sulfhydryl reagents. Table 14.3 compares the three in regard to these differences. DNA polymerase a is probably the most important for DNA replication. This enzyme shares many functional properties with DNA polymerase III of E. coli ... 

Template specificity Nicked DNA template, RNA primer Nicked DNA template, DNA primer Ribonucleotide template and DNA primer... 

RNA-dependent RNA polymerases, such as the replicases of RNA bacteriophages, are template-specific for the viral RNA. 

ProCite are currently all owned by Thomson Scientific and are available as standalone software packages for both Windows and Macintosh platforms. RefWorks is a Web-based program with individual accounts that can be accessed across platforms from any point of Internet access. Biblioscape is available in a variety of stand-alone and Web-based options. For the most part, these programs cover the gamut of research disciplines and are fairly well populated with filters and templates specific to the chemistry literature. Reviews of these and other bibliographic management software tools are regularly available in the library literature. 

ECR-1 crystallizes from a bis2(hydroxyethyl)-dimethyl ammonium (DHEDM) templated gel at temperatures above about 100°C (8), and seems to be very template specific, in the... 

There are two possible mechanisms by which a polynucleotide analog can inhibit the polymerase in the template-specific manner. Polynucleotide analogs can displace polymerase from its template and release the enzyme into solution in a free form alternatively, the polymerase can become blocked on the template polynucleotide analog complex and remain there in a bound, inactive form. These alternatives were investigated for two enzymes, alpha class DNA polymerase and E. colt RNA polymerase. In both cases the polymerase becomes blocked on the polynucleotide templates rather than being released in free form ... 

In prokaryotes, RNA is synthesized by a single kind of polymerase. In contrast, the nucleus of a eukaryote contains three types of RNA polymerase differing in template specificity, location in the nucleus, and susceptibility to inhibitors (Table 28.2). All these polymerases are large proteins, containing from 8 to 14 subunits and having a total molecular mass greater than 500 kd. RNA polymerase I is located in nucleoli, where it transcribes the tandem array of genes for 18S,... 

The experiments reported here demonstrate that the distamycin inhibition of DNA polymerase activities of FLV and MSV-M are template specific. Templates containing thymine and adenine are highly sensitive to the action of distamycins. This inhibition is dependent on the number of pyrrole rings in the molecule. The inhibition of DNA polymerases of RNA oncogenic viruses and the foci formation by distamycin derivatives conclude that both activities are dependent on the same structural component(s) of the molecule. 

The Analysis Task KB contains all kinds of analysis plan elements from abstract task components to specific tasks. Abstract components have information for plan refinement in the form of templates. Specific tasks include sampling methods, pretreatments, equipment tests, analysis methods defined in the Japan Industrial Standards and others. A specific task has information on the conditions and constraints of the task application, instructions for the task execution and so on. 

Structure— template specificity Templates forming more than one structure ... 

Structure- type Typical template Specificity Templates Structure... 

The earliest use of in vitro selection schemes to isolate RNAs with novel phenotypes from pools of mutants were those of Spiegelman et al. [83]. In these experiments replicase, a template-specific RNA-directed polymerase, was used in a Darwinian selection system to isolate a structured RNA that had reduced affinity for the intercalating dye ethidium bromide. The mutant arose by sequential mutation during the course of the experiments, and was not a preexisting variant of the original RNA pool. 

Haracska, L., Prakash, S., and Prakash, L. (2002). Yeast Revl protein is a G template specific DNA polymerase./. Biol. Chem. 277, 15546-15551. 

Table 1.1 Material Synthesis of titanium-containing zeotype and Silicon Structure Metal source source ordered mesoporous materiais Template Specific information Crystallization conditions (temperature, time) Reference (first author, year)... 

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