Problem-specific data template

Problem-Specific Data Templates. The required problem-specific data have been grouped logically and formatted into data entry templates. The first template is an interactive "control option" template used to define the structure and contents of the remaining templates. 

Once the control option template is completed, a list is displayed of all templates required for the specific problem selected. The shell at this point has defined the data required to analyze a problem. 

Most of the published studies have used probes which detect all mRNA isoforms of a given RAR or RXR. It is also possible to generate isoform-specific riboprobes by limiting the template DNA to the specifically spliced region. In situ detection of individual RAR (RXR) isoforms poses problems, however, because of limitation in the nucleotide length of isoform-specific probes (e.g., 400-500 nt in the case of RAR isoforms), and in the very low abundance of individual mRNA isoforms Nevertheless, there have been recent reports of ISH detection of RARP isoforms in chick embryos (17,18) and RARy isoforms can be specifically detected on cryosections of mouse embryos (unpublished data) Detailed procedures for cloning, amplification, and purification of plasmid DNA, which are beyond the scope of this chapter, can be found in refs. 20 and 21... 

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