Technical, Biological, and Economical Limits for Assay Miniaturization in High-Density Plates

3 Technical, Biological, and Economical Limits for Assay Miniaturization in High-Density Plates 

The overall performance of miniaturized assays is predominantly influenced by the accuracy of the involved liquid-handling processes such as aliquoting of compound solutions and consecutive liquid additions to the analyzed sample. It is the precision 

However, it has to be kept in mind that the main motivations for the application of assays based on microfluidics and microarrays are (i) their ability to enable experiments with biological samples that are only available in very small quantities (few microliters), and (ii) the reduction of the number of liquid-handling steps compared to the same assay in a well plate. These aspects are further discussed in Section 3. 

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