Tecan

The trend in the industry has been to automate the Caco-2 permeability assay using semi- or fully automated procedures. With such a system it is possible to obtain a throughput in order of approximately 400-500 compounds per week. Automated Caco-2 assay systems are commercially available through Tecan/BD Bioscience and Bohdan Mettler Toledo. In addition, automated systems for maintenance of cell cultures are commercially available, while totally automated systems for both maintenance and culturing of cells grown on permeable filter supports are under development, e.g., by companies such as The Automation Partnership. 

Saltz LB, Cox JV, Blanke C et al. Irino-tecan plus fluorouracil and leucovorin for metastatic colorectal cancer. Irinotecan Study Group. N Engl J Med 2000 343 905-914. 

Other manufacturers of PMT-based luminometers designed to read microplates include Anthos [34] and Tecan [35], which manufacture a wide range... 

MS has recently been used to measure compounds with significant levels of impurities and solubilities below the quantitation limits of other methods. Guo et al.46 described the use of LC/MS for solubility measurements in buffer solutions in a 96-well plate. Fligge et al.47 discussed an automated high-throughput method for classification of compound solubility. They integrated a Tecan robotic system for sample preparation in 384-well plates and fast LC/MS for concentration measurement. This approach is limited by LC/MS throughput. 

Allen, J. D., Biinkhuis, R. F., Wijnholds, J., and Schinkel, A. H. (1999) The mouse Bcrpl/ Mxr/Abcp gene amplification and overexpression in cell lines selected for resistance to topo-tecan, mitoxantrone, or doxorubicin. Cancer Res. 59, 4237-4241. 

Kruijtzer, C.M., Beijnen, J.H., Rosing, H., et al. (2002) Increased oral bioavadabihty of topo-tecan in combination with the breast cancer resistance protein and P-glycoprotein inhibitor GF120918. J. Clin. Oncol. 20, 2943-2950. 

Robotic Sample Processor 510 TECAN US P.O. Box 2485 Chapel Hill, North Carolina 27515,... 

The Genesis Station from Tecan The Genesis Station from Tecan (Fig. 14.3) consists of a fixed deck supporting crystallization plates and deepwell-blocks overhung by an arm capable of... 

Figure 14.3 The Genesis workstation from Tecan. (a) A general viewof the workstation. The two mobile arms move above the deck supporting the microplates, (b) A close-up view of the deck supporting the microplates, (c) The Miniprep 75 workstation is very handy when one needs to prepare crystal screening solutions from mother solutions.

The absorbance was measured on a microplate reader (Tecan, Switzerland). 

The NMR screening samples can be prepared in an automated fashion with a programmable platform such as Tecan (by Tecan) and samples can be automatically loaded into the spectrometer by using a Sample Rail (by Bruker Biospin). This allows for maximum spectrometer time and the sample is always freshly prepared prior to data acquisition. 

Pipetting robot, e. g., Genesis, and Gemini and Facts software (Tecan, Maenne-dorf, Switzerland)... 

Fig. 9.3. Pipette robot Genesis (Tecan) with integrated carousel (right).

Fig. 9.4. Control of individual modules by die Facts software (Tecan). To optimize die sequence of events, die processes are time-interlocked.

O. Wildner, R. M. Blaese, and J. C. Morris, Synergy between the herpes simplex virus tk/ganciclovir prodrug suicide system and the topoisomerase I inhibitor topo-tecan, Hum. Gene Ther. 10 2619 (1999). 

Workers at Parke-Davis reported the DIVERSOMER method in 1993 [63,64] which utilized proprietary reactors in combination with a Tecan robot. This technology has been implemented to generate libraries ofhydantoins [63-66,95], benzodiazepines [63-66, 95], and quinolones [67,96,97] by solid-phase synthesis. 

Workers at Ontogen [82,83] reported the use of the OntoBLOCK system which utilizes a Tecan robot. They have implemented this technology for solid-phase synthesis to generate over 50 000 compounds corresponding to libraries of pyrroles, phosphonates, phosphinates, lactams, imidazoles, hydantoin imides and thioimides, oxazoles, and b-lac-tams. These libraries have resulted in potent and selective inhibitors of iNOS, PTPases and cdc25 phosphatase and compounds that reverse the P-glycoprotein (Pgp)-based multiple drug resistance (MDR) phenomenon in cellular assays and in animal models [98],... 

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