TATA-less promoters

Villa-Garda M, Li L, Riely G, Bray PF Isolatimi and characterization of a TATA-less promoter for the human b3 int rin gene. Blood 83 668-676,1994. 

The promoter of STSSia I (GD3-synthase, STII), which has attracted much more attention than have other promoters of the ganglioside synthase genes, has been cloned from rat [103], mouse [104] and human [105]. The promoter of all three species is TATA-less and does not possess known core- promoter elements, including the initiator (INR), the downstream promoter (DPE), the TFIIB recognition elements (BRE), and the motif ten element (MTE). Similar to other TATA-less promoters, it has a high GC content and is enriched in Spl-bind-ing sites. The proximal promoter region is defined within 500 or 700 bp upstream of the ATG... 

Importantly, GC-rich elements also control constitutive expression of a number of other drug transporters. The MRPl promoter contains a GC-rich domain (—91 to +103) that has been shown to interact with SP-1 and is essential for its basal transcription. MRP3 features multiple GC-rich SP-1 binding sites (—91 to —21). BCRP, another TATA-less promoter, harbors several putative SP-1 binding sites, about 300 bp upstream ofthe transcription start site, which confer basal expression. 

Second group TATA-less promoters with functional initiator... 

This is the most common core promoter element A common upstream element Often found in TATA-less promoter s Octl, 0ct2 contain homei> domains... 

The pig GnT II cDNA is also on a single exon. The gene is located on chromosome Iq23-q27 and has a GC-rich TATA-less promoter with multiple transcription start sites, typical of a house-keeping gene [74]. 

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