Target tees

Where 90° turns in piping are required, standard long radius ells (ell centerline radius equals 1.5 times pipe nominal diameter) are usually used. In sandy service, the sand has a tendency to erode the metal on the outside of the bend. Target tees, such as shown in Figure 15-25, are often specified for such service. The sand builds up against the bull plug and provides a cushion of sand that is constantly being eroded and subject to deposition by the sand in the flow stream. 

The purpose/objective of the scheme, including who it is currently open to (the men tee target group). 

Manning, B. D., Tee, A. R., Logsdon, M. N., Blenis, J., and Candey, L. C. (2002). Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway. Mol. Cell 10, 151—162. 

To obtain maximal protein productivity, it is necessary to construct an expression clone in which a protein coding region (open reading frame, mature region, domain, etc.) obtained from a cDNA of interest is inserted into the MCS of the pTD 1 vector. Typically, expression of the target protein at about 35-50 pg per mL of the translation reaction mixture can be obtained by using mRNA transcribed from the expression clone and the Transdirect insect cell kit. Furthermore, the expression clone can be effectively combined with other eukaryotic cell-free protein synthesis systems, such as rabbit reticulocyte lysate and wheat germ systems (tee Note 3). 

After linearization of the transfer vector with alkaline phosphatase, the target gene DNA is ligated into the linearized transfer vector by using DNA Ligation Kit Ver 2.1 (TAKARA BIO INC., Japan) (tee Note 5). 

A new class of antiasthma drugs has recently been developed that targets the formation or function of leukotrienes (Figure 22.7). These drugs include zileuton [ZYE loo ton], zafirlukast [zah FUR loo kast], and montelukast [mon TEE loo kast]. 

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