Target identification system libraries

Finally, we have recently successfully applied MASPIT to the screening of cDNA libraries and to the identification of novel small molecule-protein interactions [64], These studies mark the beginning of the development of a broadly applicable M3H system that holds promise for future use in target identification and drug discovery. 

Genetics AcB Randomly perturbate the system looking for changes in a specific phenotype Target/pathway identification Assemble large diverse chemical library Assay for specific phenotype Identify targets of compounds that induce the phenotype... 

If a system was returned from inspection, external exercise, or maintenance at the manufacturer, an additional test for cleanness (entrance control) is performed. Three different blank injections with solvent, BSTFA, and DMT are performed and evaluated using an on-site target library containing all MS spectra from the OCAD. Absence of any identification ensures that the system components are free from scheduled chemicals and their degradation products. The same blank injection tests are performed if a system in waiting status was used internally for injections other than check mixture. The on-site target library used in the test of cleanness is removed from the hard disk before issuing the system for a mission. 

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