Tandem mass spectrometry biomarker identification

Melle, C., Ernst, G., Schimmel, B., Bleul, A, Koscielny, S., Wiesner, A., Bogumil, R., Moller, U., Osterloh, D., Halbhuber, K.J., and F. Von Eggling, 2003, Biomarker Discovery and Identification in Laser Microdissected Head and Neck Squamous Cell Carcinoma with ProteinChip(R) Technology, Two-dimensional Gel Electrophoresis, Tandem Mass Spectrometry, and Immunohistochemistry. Mol Cell Proteomics. 2(7) 443-52. 

H.X. Wang, M.T. Kachman, D.R. Schwartz, K.R. Cho and D.M. Lubman, Comprehensive proteome analysis of ovarian cancers using liquid phase separation, mass mapping and tandem mass spectrometry a strategy for identification of candidate cancer biomarkers, Proteomics, 4, 2476-2495 (2004). 

This chapter describes a step-by-step protocol for top-down proteomic identification of protein biomarkers of bacterial food-borne pathogens using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and web-based software developed at the Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture (USDA). 

Fagerquist, C.K., WiUiams, K.E., Bates, A.H. (2007) Identification of foodborne bacteria by high energy collision-induced dissociation of their protein biomarkers by MALDI tandem-time-of-flight mass spectrometry. In Proceedings of the 55th American Society of Mass Spectrometry Conference, June 3-7, Indianapolis, IN [DVD MPT-327 from ASMS]. 

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