Tandem mass spectrometric strategy

Hogenboom A.C., Niessen W.M.A., and Brinkman U.A., 1999a. Online solid-phase extraction-short-column liquid chromatography combined with various tandem mass spectrometric scanning strategies for the rapid study of transformation of pesticides in surface water. J Chromatogr A 841 33. 

Tiller, PR. Romanyshyn, L.A. Liquid Chromatography/Tandem Mass Spectrometric Quantification with Metabolite Screening as a Strategy to Enhance the Early Drug Discovery Process, Rapid Commun. Mass Spectrom. 16(12), 1225-1231 (2002). 

Tiller PR and Romanyshyn LA (2002) Liquid chromatography/tandem mass spectrometric quantification with metabolite screening as a strategy to enhance the early drug discovery process. Rapid Commun Mass Spectrom 16 1225-1231. 

It has been demonstrated also that the iTRAQ tandem mass spectrometric quantitative analysis strategy can be used in conjunction with the quadrupole ion trap by performing multiple stages of mass analysis (that is, MS ) [125], For example, chemical derivatization with the iTRAQ reagent not only labels the N-terminus of a peptide, but the lysine side chain also. Thus, tryptic peptides with a modified lysine residue present at the C-terminus will produce a yj product ion at m/z 291 following ClD-tandem mass spectrometry. To generate the low m/z iTRAQ reporter ions required for quantitation, the yj product ion is isolated and subjected to data-dependent CID-MS. Using this approach, peptide identification is achieved in the MS/MS scan, while quantitation is achieved via MS. ... 

Chemical derivatization methods provide a useful additional tool for protein structural analysis, particularly when conpled with the multistage tandem mass spectrometric capabilities of modern ion trap mass spectrometers. The objective of this chapter was to provide a brief overview of the chemical derivatization strategies that are employed currently to address the challenges associated with protein identification, characterization, and quantitative analysis as well as for the characterization of protein-protein interactions. 

Improvements in analytical capability for the analysis of complex pyrolysate mixtures have appeared during the last decade high-resolution capillary GC with more polar and selective stationary phases coated on inert fused-silica colmnns coupling of capillary GC with sensitive, selective, and lower-cost mass spectrometric detectors enhanced pyrolysis-MS techniques hyphenated analysis methods, including GC-Fourier-transform infrared spectroscopy (GC/FTIR) and tandem MS and better strategies for handling complex multidimensional pyrolysis data. The present chapter reviews the known chemotaxonomy of miCTOorganisms, summarizes practical considerations for the use of pyrolysis in microbial characterization, and critically discusses selected applications of analytical pyrolysis to microbial characterization. 

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