Synthetic gene construction

Genetic Engineering and Microbial Biosyntheses Synthetic Gene Constructions and Expression in E. coli... 

Lyons, R.E., Lesieur, E., Kim, M., Wong, D., Brownlee, A., Pearson, R., and Elvin, C., The development of synthetic genes encoding repetitive resilin-like polypeptides Construct design, bacterial expression and rapid purification. Protein Eng. Design Select., 20(1), 25-32, 2007. 

FIGURE 3.11 Construction of a synthetic gene from chemically synthesized oligonucleotides. 

The gene constructs used are synthetic constructs. The introduction of pure DNA would lead to its rejection by the receiving organism. The production of synthetic constructs is needed to have any effect. Whether they work or not is tested in a trial and error process. 

Tcf-1 and Lef-1 are not classical transcription factors in that they are incapable of activating transcription of reporter gene constructs from a synthetic enhancer containing multimerized Tcf/Lef binding sites. Lef-1 acts as... 

Prince, J.T. McGrath, K.P. DiGirolamo, C.M. Kaplan, D.L. Construction, cloning and expression of synthetic genes encoding spider dragline silk. Biochemistry 34 10879-10884 (1995). 

To construct the synthetic gene in a vector suitable for future mutagenesis, plasmid pWR13 was modified a synthetic polylinker Ecorl-Xbal shown in Fig.2 was used to replace the polylinker... 

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