Construction of Synthetic Genes

Nat Protoc Exchange http //www.nature.com/protocolexchange/protocols/561 Ellis T, Wang X, Collins JJ (2009) Diversity-based, model-guided construction of synthetic gene networks with predicted functions. Nat Biotechnol 27 465-471 Ellis T, Adie T, Baldwin GS (2011) DNA assembly for synthetic biology from parts to pathways and beyond. Integr Biol 3 109-118... 

Sandhu GS, Aleff RA and Kline, BC (1992) Dual asymmetric PCBj One-step construction of synthetic genes. Biotechniques 12 14-16. 

DiUon PJ, Rosen CA (1990) A rapid method for the construction of synthetic genes using the polymerase chain reaction. Biotechniques 9 298-300. 

Lyons, R.E., Lesieur, E., Kim, M., Wong, D., Brownlee, A., Pearson, R., and Elvin, C., The development of synthetic genes encoding repetitive resilin-like polypeptides Construct design, bacterial expression and rapid purification. Protein Eng. Design Select., 20(1), 25-32, 2007. 

Biotransformations, bioconversions impact on the whole range of biological, biotechnological and biomedical applications, such as in the analysis of genes and transcripts, the therapeutic treatment of abnormal molecules in diseases, the construction of specific enzymes and proteins for therapy, analysis, production, the assembly and construction of synthetic bio-synthetic pathways in organisms for the synthesis of intermediates, antibiotics, vitamins, enzymes, flavors. 

Prince, J.T. McGrath, K.P. DiGirolamo, C.M. Kaplan, D.L. Construction, cloning and expression of synthetic genes encoding spider dragline silk. Biochemistry 34 10879-10884 (1995). 

FIGURE 3.11 Construction of a synthetic gene from chemically synthesized oligonucleotides. 

The gene constructs used are synthetic constructs. The introduction of pure DNA would lead to its rejection by the receiving organism. The production of synthetic constructs is needed to have any effect. Whether they work or not is tested in a trial and error process. 

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