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Synthetic gene construction transgene expression

The potential use of spider silk as a new biomaterial has led to the evaluation of various heterologous expression systems for the production of recombinant spider silk-like proteins [65]. Partial cDNA constructs of dragline silk protein were cloned and expressed in Escherichia coli [66], mammalian cell lines (MAC-T/bovine and BHK (baby hamster kidney)/hamster) [67], insect-cell lines [68, 69], and transgenic silkworm larvae [69]. Designer synthetic genes based on Nephila clavipes spider dragline and fiagdliform protein sequences have also been expressed in E. coli... [Pg.194]

See other pages where Synthetic gene construction transgene expression is mentioned: [Pg.35]    [Pg.398]    [Pg.270]    [Pg.103]    [Pg.5]    [Pg.375]    [Pg.383]    [Pg.48]    [Pg.814]    [Pg.419]    [Pg.176]    [Pg.334]    [Pg.367]    [Pg.367]    [Pg.945]    [Pg.443]   


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