Synechocystis PCC

A shift in temperature from 38 to 22 °C leads to desaturation of fatty acids in Anabaena variabilis [110], resulting in control of the fluidity of the plasma membrane. Mutants have been isolated in Synechocystis PCC 6803 that were defective in desaturation of fatty acids, and the growth rate of one of these mutants was much lower than that of the wild-type at 22 °C [112]. It turned out that the mutant strain had a mutation in the gene desA, and when the wild-type allele was introduced into the chilling-sensitive cyanobacterium Anacystis nidulans, it resulted in increasing the tolerance of that strain to low temperature [113]. These experiments nicely demonstrate the existence of a mechanism of adaptation to low temperature in a chilling-tolerant cyanobacterium. 

El Bissati, K., E. Delphin, N. Murata, A. Etienne, and D. Kirilovsky (2000). Photosystem II fluorescence quenching in the cyanobacterium Synechocystis PCC 6803 Involvement of two different mechanisms. Biochim Biophys Acta 1457(3) 229-242. 

Disch, A., Schwender, J., Muller, C., Lichtenthaler, H.K., and Rohmer, M., Distribution of the mevalonate and glyceraldehyde phosphate/pyruvate pathways for isoprenoid biosynthesis in unicellular algae and the cyanobacterium Synechocystis PCC 6714, Biochem.., 333, 381, 1998. 

There have been few studies to date of the functionality and stability of AP-trapped photosynthetic reaction centers. Rhodobacter sphaeroides reaction centers were shown to remain intact following trapping with AP A8-75 (a more highly charged analog of A8-35), but neither their functionality nor their stability over time were studied[5]. Synechocystis PCC 6803 PS1 reaction centers trapped with A8-35 and deposited on a gold electrode have been shown to be electrochemically active, but their long-term stability has not been studied[12]. The photochemical activity of A8-35-trapped pea PS2 reaction centers, measured at room temperature by the accumulation of the pheophytin free radical upon illumination, was found to be intermediate between that in chaps and in P-DM solutions [A. Zehetner H. Scheer, personal communication ref. 13],... 

Fuhs et al.m investigated P p0 Aj in multilayers of Synechocystis PCC 6803 oriented on mylar sheets by transient W-band EPR. They could show an enhanced resolution of structural parameters of the RP in this model system. A problem is the uncertainty of the orientation distribution (width 30 10°). Limitations and possibilities of the method are discussed in this work. The technique is interesting for all systems for which no single crystals are available. 

Creuzet, S., G. Ajlani, C. Vernotte, and C. Astier (1990). A new ioxynil-resistant mutant in Synechocystis PCC 6714 Hypothesis on the interaction of ioxynil with the D1 protein. Z. Naturforsch. Sect. CBiosci., 45 436 -40. 

Jakopitsch C, Vlasits J, Wiseman B et al (2007) Redox intermediates in the catalase cycle of catalase-peroxidases from Synechocystis PCC 6803, Burkholderia pseudomallei, and Mycobacterium tuberculosis. Biochemistry 46 1183-1193... 

Boichenko, V.A., Hou, J-M., and Mauzerall, D. (2001) Thermodynamics of electron transfer in oxigen photosynthetic reaction centers volume change, enthalpy and entropy of electron-transfer reactions in intact cells of the cyanobacterium Synechocystis PCC 6803, Biochemistry 40, 7126-7132. 

Chatterjee, A., Majee, M., Ghosh, S., and Majumder, A.L., 2004, slll722, an unassigned ORF of Synechocystis PCC 6803, codes for L-myo-Inositol 1-Phosphate Synthase. Planta 218 989-998. 

Florencio, F. J., Marques, S., and Candau, P. (1987). Identification and characterization of a glutamate dehydrogenase in the unicellular cyanobacterium Synechocystis PCC 6803. FEBS Lett. 223, 37-41. Flores, E., and Herrero, A. (1994). Assimmilatory nitrogen metabolism and its regulation. In The Molecular Biology of Cyanobacteria (Bryant, D. A., ed.). Kluwer Academic, Dordrect, The Netherlands, pp. 487-517. 

M Herv/Es, JM Ortega, JA Navarro, MA De la Rosa and H Bottin (1994) Laserflash kinetic analysis of Synechocystis PCC 6803 cytochrome Cg and plastocyanin oxidation by photosystem I. Biochim Biophys Acta 1184 235-241... 

A C4-RP HPLC method coupled to ESI-MS has been described for the analysis of the different components of phycobilisome from the cyanobacterium Synechocystis PCC 6803 (Zolla and Bianchetti, 2001). 

Zolla, L. and Bianchetti, M. 2001. High-performance liquid chromatography coupled on-line with electrospray ionization mass spectrometry for the simultaneous separation and identification the Synechocystis PCC 6803 phycobihsome proteins. J. Chromatogr. 912A, 269-279. 

Kanervo E, Maenpak P, Aro EM. Dl protein degradation and psbA transcript levels in Synechocystis PCC 6803 during photoinhibition in vivo. J Plant Physiol 1993 42 669-675. 

Komenda J, Lupinkovd L, Kopecky J. Absence of the psbFI gene product destabilizes photosystem II complex and bicarbonate binding on its acceptor side in Synechocystis PCC 6803. Eur J Biochem 2002 269 610-619. 

Lupfnkovd L, Metz JG, Diner BA et al. Histidine residue 252 of the Photosystem II D1 polypeptide is involved in a light-induced cross-linking of the polypeptide with the a-subunit of cytochrome b-559 Study of a site-directed mutant of Synechocystis PCC 6803. Biochim Biophys Acta 2002 1554 192-201. 

Fig. 2. Ptiototactic movements of colonies of the slr1694 gene-disrupted mutant (Aslr1694) and its parent strain, Synechocystis PCC-P. 1 p.l of each cell suspension was spotted and grown for 44 h under lateral illumination arrow) at 460 nm (left) and 660 nm (right). The dotted line shows the initial position of inoculation before the illumination. Reprinted with permission from ref. 39.

X. X., and Ikeuchi, M. (2000) Novel putative photoreceptor and regulatory genes required for the positive phototactic movement of the unicellular motile cyanobacterium Synechocystis PCC 6803. Plant Cell Physiol. 41, 1299-1304. 

Introduction of foreign DNA into cyanobacteria has been demonstrated in laboratory for several strains, and is now a common practice [50]. A few unicellular cyanobacteria are naturally competent for transformation, and can uptake foreign DNA from their environment in the form of plasmid or linear DNA [51]. Among naturally competent strains are the model freshwater cyanobacteria S. elongatus PCC 7942 and Synechocystis PCC 6803, as well as the marine Synechococcus PCC 7002 and the thermophile Jhermosynechococcus elongatus BP-1 [52—56]. 

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