Anacystis nidulans

Figure 18.4 The hanging-drop method of protein crystallization, (a) About 10 pi of a 10 mg/ml protein solution in a buffer with added precipitant—such as ammonium sulfate, at a concentration below that at which it causes the protein to precipitate—is put on a thin glass plate that is sealed upside down on the top of a small container. In the container there is about 1 ml of concentrated precipitant solution. Equilibrium between the drop and the container is slowly reached through vapor diffusion, the precipitant concentration in the drop is increased by loss of water to the reservoir, and once the saturation point is reached the protein slowly comes out of solution. If other conditions such as pH and temperature are right, protein crystals will occur in the drop, (b) Crystals of recombinant enzyme RuBisCo from Anacystis nidulans formed by the hanging-drop method. (Courtesy of Janet Newman, Uppsala, who produced these crystals.)...

A shift in temperature from 38 to 22 °C leads to desaturation of fatty acids in Anabaena variabilis [110], resulting in control of the fluidity of the plasma membrane. Mutants have been isolated in Synechocystis PCC 6803 that were defective in desaturation of fatty acids, and the growth rate of one of these mutants was much lower than that of the wild-type at 22 °C [112]. It turned out that the mutant strain had a mutation in the gene desA, and when the wild-type allele was introduced into the chilling-sensitive cyanobacterium Anacystis nidulans, it resulted in increasing the tolerance of that strain to low temperature [113]. These experiments nicely demonstrate the existence of a mechanism of adaptation to low temperature in a chilling-tolerant cyanobacterium. 

A 2-amino-2-deoxyheptose of unknown configuration is a minor component of the LPS from the photosynthetic procaryote Anacystis nidulans. ... 

Gombos, Z., M. Kis, T. Pali, and L. Vigh. 1987. Nitrate starvation induces homeoviscous regulation of lipids in the cell envelope of the blue-green alga, Anacystis nidulans. Eur. J. Biochem. 165 461—465. 

Phycochrome a, extracted from Tolypothrix distorta, Phormidium luridum, Nostoc muscorum and Anacystis nidulans, has one form absorbing maximally at about 590 nm, formed in red light, and another one absorbing at 570 nm, formed in yellow-green and blue-green light. 

Lee, L.H. and B. Lustigman. 1996. Effect of barium and nickel on the growth of Anacystis nidulans. Bull. Environ. Contam. Toxicol. 56 985-992. 

Whitton, B.A. and F.H.A. Shehata. 1982. Influence of cobalt, nickel, copper and cadmium on the blue-green alga Anacystis nidulans. Environ. Pollut. 27A 275-281. 

Kumar, H.D. and G. Prakash. 1971. Toxicity of selenium to the blue-green algae, Anacystis nidulans and Anabaena variabilis. Ann. Bot. 35 697-705. 

Boison, G., Schmitz, O., Mikheeva, L., Shestakov, S. and Bothe, H. (1996) Cloning, molecular analysis and insertional mutagenesis of the bidirectional hydrogenase genes from the cyanobacterium Anacystis nidulans. FEES Lett., 394, 153-8. 

Peschek, G. A. (1979a) Evidence for two functionally distinct hydrogenases in Anacystis nidulans. Arch. Microbiol., 123, 81-92. 

Peschek, G. A. (1979b) Aerobic hydrogenase activity in Anacystis nidulans. The oxyhydrogen reaction. Biochim. Biophys. Acta, 548, 203-15. 

Blue-green alga, Anacystis nidulans, boric acid,... 

Martinez, E, P. Matio, I. Bonilla, and E. Fernandez-Valiente. 1986a. Cellular changes due to boron toxicity in the blue-green Anacystis nidulans. Phyton 46 145-152. 

Martinez, E, P. Mateo, I. Bonilla, E. Femandez-Valiente, and A. Garate. 1986b. Growth of Anacystis nidulans in relation to boron supply. Israel Jour. Bot. 35 17-21. 

The three-dimensional structures, or part of it, are also known for Desulfovibrio vulgaris and Anacystis nidulans flavodoxins. These results, including those obtained on C.MP., were recently summarized by Adman . Hence, these results will be discussed only briefly. The x-ray structures show that the isoalloxazine ring is embedded in a hydrophobic pocket of the apoprotein, i.e. flanked by at least one aromatic amino acid residue. During the redox transitions, especially from the oxidized to the semiquinone state, small conformational changes occur and contacts with the isoalloxazine ring are formed or broken. These conformational transitions form probably a kinetic barrier so that the semiquinone state is trapped by the apoprotein and, therefore, rather stable towards oxidation by molecular oxygen. 

Fig. 4. Dendrogram of Q, C4, and CAM-PEPCase sequences. Amino acid sequences were deduced from either genomic or cDNA sequences. An, Anacystis nidulans Cg, Corynebaclerium glutamicus Ec, Escherichia coli Nt, Nicotiana tabacum, Q-form Mcl, Mesembryan-themum crystattinum, CAM-form Mc2, Mesembryanthemum crystal-linum, C3-form Sv, Sorghum vulgaris, C4-form Zm, Zea mays, C4-form. For references see the text.

Katagiri, F., Kodaki, T., Fujita, N., Izui, K., Katsuki, H. (1985). Nucleotide sequence of the phosphoeno/pyruvate carboxylase gene of the cyanobacterium Anacystis nidulans. Gene 38, 265-9. 

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