Synechococcus cultures

Fig. 2.125. HPLC chromatograms generated at 440 nm. Pure cultures (a) Synechococcus elongatus, (b) 2 micron picosphere , (c) Cyclotella choctawatcheena. Phytoplankton field communities (d) Twin Key Basin, (e) Rabbit Key Basin, (f) Sandy Key Basin, ft = /1-carotene, Z = zeaxanthin, a = chlorophyll-a, N = myxoxanthophyll, E = echinenone, c,/c2 = chlorophylls-Cj/c2, F = fucoxanthin, Col = unknown carotenol, Cone = unknown carotenone, D = diadinoxan-thin, P = peridin. Reprinted with permission from J. W. Louda et al. [283].

Marie, D., Bmssaard, C. P. D., Thyrhaug, R., Bratbak, G., and VarJot, D. (1999). Enumeration of marine viruses in culture and natural samples by flow cytometry. Appl. Environ. Microbiol. 65, 45—52. McDaniel, L., and Capone, D. G. (1985). A comparison of procedures for the separation of aquatic bacteria from sediments for subsequen direct enumeration. J. Microbiol. Methods 3, 291—302. McDaniel, L., Houchin, L. A., Williamson, S. J., and Pard, J. H. (2002). Lysogeny in marine Synechococcus. Nature 415, 496. 

Figure 38.2 A progressive shift from a Synechococcus-dominated community to a diatom-dominated assemblage with increasing Fe supply in an experiment in the Peru Upwelling region. A shipboard continuous culture system was used to supply a continuous flow of Fe-amended seawater at adilution rate of 1 day simulating upwelling of water with three different Fe concentrations. Iron supply rates used were 0.8,1.3, or 3.7 nmol Fe L day . Values are the means and error bars are the standard deviations of triplicate incubation bottles. Redrawn from Hare et al. (2005), Aquatic Microbial Ecology 38 269—282.

Liquid cultures of Synechocystis PCC 6714 and Synechococcus PCC 6301 were maintained under log growth in continuous culture turbidostats, then transferred at a chlorophyll (Chi) concentration of 5 ig/ml to glass test tubes of 25 mm diameter in standard growth medium for experimental treatments (details in [2]). Cells were photoinhibited by illumination at a photon flux density of 1220 iE m s" at the cell surface. For Ca + or Na+ depletion experiments the cells were suspended in otherwise... 

Fig. 2. Histograms showing relative levels of individual psbA transcripts in wild-type and mutant strains of Synechococcus following transfer of cells to different culture PPFD for 15 minutes.

Growth of bacteria, manipulation of plasmids pUC18 and pHP45D, and Southern blot analysis were carried out as previously described (11,12,13). Cultures of Synechococcus PCC 7942 were maintained on BG-11 medium and grown in batch culture at 28 C in an illuminated shaker (14). Isolation of... 

FIGURE 1. Intracellular inorganic carbon accumulation and CO2 fixation in Synechococcus PCC7942 (wild), C3P-0 (mutant) and transformants determined by silicone oil centrifugation method. Culture and assay were performed at 41 C. NaH C03 (50... 

The main elements within the mineral (or ash) fraction include P, Ca, Na, K, Cl, Fe, Mg and, for diatoms. Si (Fabregas and Herrero, 1986). Species can differ significantly in their composition of these minerals and other trace metals moreover, their composition is affected by culture environment (Lee and Picard, 1982). For example, Synechococcus sp. cells accumulated ions (Co, Zn, Ag, Sn, Hg, Pu and Am) in proportion to the concentration of the same ions in culture media (Fisher, 1985). 

Synechoxanthin, an aromatic xanthophyll has recently been isolated with a very high yield (15% of the total carotenoids) in the culture medium of the euryhaline cyanobacterium Synechococcus sp. strain PCC 7002. This discovery suggests that cyanobacteria are able to synthesize aromatic carotenoids (Graham et al., 2008). 

Synechococcus sp. Miami BG043511 was isolated from the sample collected from marine environment of Bahama islands and purified to axenic status in this laboratory (Rosner, Radway, Sprogis, Duerr, Leon, Mitsui, Unpublished). This blue-green alga was grown under aseptic conditions in combined nitrogen free medium A-N (Kumazawa, Mitsui, 1981) in 4 liter aspirator bottles at 25oC and 100/x Einsteins/m /sec. Culture was bubbled with sterile air. 

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