Surface swabs

Surface Swab Rinse Swab Rinse Swab Rinse ... 

The use of contact plates is the recommended technique. The RODAC plate method is the simplest, but it is useful only for flat surfaces. Swab samples afford examination of corners, crevices, and other area inaccessible to RODAC plates. 

Sampling Technique Calculations for Surface Swabs Calculation for Final Rinse Sample... 

Attachment No. 2200.40(D) Sampling Technique Attachment No. 2200.40(E) Calculations for Surface Swabs... 

Stainless steel surfaces swabbed with trichloethane immediately before insertion in the XPS spectrometer, consistently showed evidence of chloride retention in their Cl(2p) spectra, even after exposure to a spectrometer vacuum of 10 Pa for 2A hours at 3A-A0°C. Figure 5(a) shows such a spectrum. From the Cl(2p) spectral envelope, two sets of Cl (2p 3/2-1/2) doublets were derived by deconvolution. The more intense doublet "1" is assigned to organically-bound chlorine, being close in energy to chlorine bound in some aliphatic hydrocarbons. The Cl(2p 3/2) line labelled "2" corresponds closely to the binding energies. . ... 

Korfinacher WA, Rushing LG, Nestorick DM, et al. 1985. Analysis of dust and surface swab samples for octachlorodibenzo-p-dioxin and heptachlorodibenzo-p-dioxins by fused silica capillary GC with EC detection. Journal of High Resolution Chromatography and Chromatography Communications 8 12-19. 

Accuracy / recovery The amount of material recovered from surface, swab or rinse solution compared to accepted reference value. A correction factor should be established in the analytical method to represent incomplete recovery + +... 

Air samples Surface swabs Operator swabs Daily clothing... 

It is vital that microbial contamination of clean and aseptic areas should not exceed acceptable limits. Such areas should be monitored for microbial contamination by such methods as seme plates, air samplers and surface-swabs, etc. 

FIGURE 25.9 A comparison of (a) a mixed DNA profile (two females and one male contributor) followed by (b) the Y-STR profile of the same sample. The sample was collected with a single surface swab (moistened with DI water) from a knife handle (homicide case). Amplification was performed using PowerPlex Y with 1.2 ng and Profiler Plus with 0.8 ng of template DNA. The male component DNA obtained from the knife handle and the suspect has the same Y-haplotype therefore, the suspect could not be eliminated as the source of the male DNA in the mixture. The results also indicate the presence of only one male contributor in the DNA extract. It should be noted that all of the suspect s male relatives from his paternal lineage would also have the same Y-haplotype. (Courtesy of DNA Unit—Orange County Sheriff-Coroner Department, CA.)... 

GRAM L, MELCHIORSEN J and BRUHN I B (2010) Antibacterial activity of marine cultur-able bacteria collected from a global sampling of ocean surface waters and surface swabs of marine organisms, 7 Mar Biotechnol, 12,439-451. 

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