Surface plasmon resonance interpretation

Pharmacokinetic data analysis requires determination of the analyte in various body fluids. In the case of therapeutic antibodies, serum is the most common matrix to be analyzed. For a critical interpretation of pharmacokinetic data the chosen bioanalytical methods must be considered. The most frequently used for mAbs include enzyme-linked immunosorbent assay (ELISA), capillary electrophoresis (CE)/polyacrylamide gel electrophoresis (PAGE), fluorescence-activated cell sorting (FACS), and surface plasmon resonance (SPR). The challenges and limitations of bioanalytical methods used for the analysis of mAb concentrations are discussed in detail in Chapter 6. 

A chemical sensor is a device that transforms chemical information into an analytically useful signal. Chemical sensors contain two basic functional units a receptor part and a transducer part. The receptor part is usually a sensitive layer, therefore a well founded knowledge about the mechanism of interaction of the analytes of interest and the selected sensitive layer has to be achieved. Various optical methods have been exploited in chemical sensors to transform the spectral information into useful signals which can be interpreted as chemical information about the analytes [1]. These are either reflectometric or refractometric methods. Optical sensors based on reflectometry are reflectometric interference spectroscopy (RIfS) [2] and ellipsometry [3,4], Evanescent field techniques, which are sensitive to changes in the refractive index, open a wide variety of optical detection principles [5] such as surface plasmon resonance spectroscopy (SPR) [6—8], Mach-Zehnder interferometer [9], Young interferometer [10], grating coupler [11] or resonant mirror [12] devices. All these optical... 

Jung LS, Campbell CT, Chinowsky TM et al (1998) Quantitative interpretation of the response of surface plasmon resonance sensors to adsorbed films. Langmuir 14 5636-5648... 

Jung, L. S., Campbell, C. T., Chinowsky, T. M., et al. (1998) Quantitative Interpretation of the Response of Surface Plasmon Resonance Sensors to Adsorbed Films. Langmuir 14 5636-5648. 

In this chapter the influence of the structural parameters on the optical behavior of silver nanoparticles is anal3fzed. The absorption and scattering spectra are obtained for particles with different size and shape in the framework of the discrete dipole approximation. Radially symmetric nanoparticles, as well as finite-number faces nanoparticles or multi-tips objects are investigated under the excitation of uniform fields impacting with different poiarizations and propagation directions. The optical responses can be assigned to the excitation of iocalized surface plasmon resonances of different order. The presented results can be used to interpret experimental measurements and/or to develop new high-performance substrates for molecular plasmonics applications. 

One attractive feature of host templates such as porous anodic alumina is the parallel alignment of the pores. This feature permits the straightforward interpretation of the plasmon resonance spectra of non-spherical metal particles that are prepared within such pores. Figure 16.2.7A shows the plasmon resonance spectra of a series of gold nanorod/ porous alumina composite films measured with light incident normal to the film surface (23, 84). 

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