Surface-enhanced laser desorption ionization technology

Dare TO, Davies HA, Turton JA, et al. Apphcation of surface-enhanced laser desorption/ionization technology to the detection and identification of urinary parvalbumin-alpha a biomarker of compoimd-induced skeletal muscle toxicity in the rat. Electrophoresis 2002 23 3241-51. 

Wright, G. L., Cazares, L. H., Leung, S.-M., Nasim, S., Adam, B.-L., Yip, T.-T., Schellhammer, P. F., Gong, L., and Vlahou, A. (2000). ProteinChip surface enhanced laser desorption/ionization (SELDI) mass spectrometry a novel protein biochip technology for detection of prostate cancer biomarkers in complex protein mixtures. Prostate Cancer and Prostatic Diseases 2, 264-276. 

The ProteinChip System from Ciphergen Biosystems uses patented SELDI (Surface-Enhanced Laser Desorption/Ionization) ProteinChip technology to rapidly perform the separation, detection, and analysis of proteins at the femtomole level directly from biological samples. ProteinChip Systems use ProteinChip Arrays which contain chemically (cationic, anionic, hydrophobic, hydrophilic, etc.) or biochemically (antibody, receptor, DNA, etc.) treated surfaces for specific interaction with proteins of interest. Selected washes create on-chip, high-resolution protein maps. This protein mass profile, or reten-tate map of the proteins bound to each of the ProteinChip Array surfaces, is quantitatively detected in minutes by the ProteinChip Reader. 

Mian S, Ball G, Hornbuckle J, et al. A prototype methodology combining surface-enhanced laser desorption/ionization protein chip technology and artificial neural network algorithms to predict the chemoresponsiveness of breast cancer cell lines exposed to Paclitaxel and Doxorubicin under in vitro conditions. Proteomics 2003 3(9) 1725-1737. 

Surface-enhanced laser/desorption ionization mass spectrometry Protein-Chip . See Tang, N., Tomatore, P, and Weinberger, S.R., Current developments in SELDI affinity technology. Mass Spectrom. Rev. 23a, 34-44,2004. 

Wright, G.L. Cazares, L.H. Leung, S.M. et al. ProteinChip Surface Enhanced Laser Desorption/ionization (SELDI) Mass Spectrometry A Novel Protein Biochip Technology for Detection of Prostate Cancer Biomarkers in Complex Protein Mixtures, Prostate Cancer and Prostatic Dis., 2, 264-276 (2000). 

V. Seibert, A. Wiesner, Th. Buschmann, J. Meuer, Surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDl TOF-MS) and ProteinChip technology in proteomics research. Pathol. Res. Practice 200, 83 (2004)... 

In 1993, Hutchens and co-workers described surface-enhanced laser desorption/ionization (SELDI) technique, an affinity technology, which has progressed over the last decade to become a powerful analytical, an on-plate approach (Hutchens and Yip 1993). SELDI is a distinctive form of laser desorption/ionization (LDI) mass spectrometry in which the EDI probe plays an active role in the homogenization, preconcentration, amplification, purification, extraction, enrichment digestion, derivatization, synthesis, separation, and detection with complementary techniques, prior to the desorption and ionization of the analytes by MALDI (Merchant and Weinberger 2000). The principle of this approach is very simple. Biomolecules are captured by adsorption, partition, electrostatic interaction, or affinity chromatography on a solid-phase protein chip surface. Although SELDI provides a unique sample preparation platform, it is similar to MALDI-MS in that a laser... 

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