Sulfate-reducing bacteria hydrogenases

Sulfate reducing bacteria of the genus Desulfovibrio are one of the main sources of enzyme. Hydrogenases can be found in different sites in the bacterial cell periplasm, cytoplasm, and membrane. A given species may have hydrogenases in one or in several of these cell sites. 

The hydrogenases isolated can be broadly classified into two groups [Fe-S]-only, and nickel-[Fe-S]-containing hydrogenases. The first group, the [Fe-S]-only or [Fe] hydrogenases, is characterized by the absence of nickel on the active site, and are rare in sulfate reducing bacteria. They contain [4Fe-4S] clusters and, in addition, a catalytic... 

Moura, J. J. G., Teixeira, M., Moura, I. and LeGall, J. (1988) (Ni,Ee)-hydrogenases from sulfate-reducing bacteria Nickel catalytic and regulatory roles. In J. R. Lancaster (ed.). Bioinorganic Chemistry of Nickel. Deerfield Beach Florida 91226, VCH Publishers. 

Odom, J. M. and Peck, H. D. (1984) Hydrogenase, electron-transfer proteins, and energy coupling in the sulfate reducing bacteria Desulfovibrio. Ann. Rev. Microbiol., 38, 551. 

Fauque G, Peck HD Jr, Moura JJG, et al. 1988. The three classes of hydrogenases from sulfate-reducing bacteria of the genus Desulfovibrio. FEMS Microbiol Rev 54 299-344. 

To the memory of Harry D. Peck, Jr. (1927-1998) professor, founder, and chairman of the Department of Biochemistry at the University of Georgia and pioneer in studies of sulfate-reducing bacteria and hydrogenases. 

Steuber J, Cypionk H, Kroneck PMH (1994) Mechanism of dissimilatory sulfite reduction by Desulfovibrio desulfuricans purification of membrane-bound sulfite reductase and coupling with cytochrome c3 and hydrogenase. Arch Microbiol 162 255-260 Stille W, Triiper HG (1984) Adenylylsulfate reductase in some new sulfate reducing bacteria. Arch Microbiol 137 145-150... 

Heterodimeric [NiFe]-hydrogenase crystal structures have been reported for four closely related sulfate-reducing bacteria from Desulfovibrio sp. D. gi-gas [40,41], D. vulgaris (Miyazaki) [42 - 44], D. fructosovorans [45,46] and D. desulfuricans [47]. Overall, the structures are very similar being roughly... 

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