Sulfate-reducing bacteria, hydrogenases from

Moura, J. J. G., Teixeira, M., Moura, I. and LeGall, J. (1988) (Ni,Ee)-hydrogenases from sulfate-reducing bacteria Nickel catalytic and regulatory roles. In J. R. Lancaster (ed.). Bioinorganic Chemistry of Nickel. Deerfield Beach Florida 91226, VCH Publishers. 

Fauque G, Peck HD Jr, Moura JJG, et al. 1988. The three classes of hydrogenases from sulfate-reducing bacteria of the genus Desulfovibrio. FEMS Microbiol Rev 54 299-344. 

Several crystal structures of [NiFe] hydrogenases have been determined from sulfate-reducing and photosynthetic bacteria [8, 84, 85], and recently also from oxygen-tolerant species [9, 10]. Two structures from the subclass [NiFeSe] hydrogenase are known [86-88] and from two [FeFe] hydrogenases [8, 89, 90],... 

Like pesticides, heavy metals are traditionally tested by enzyme inhibition or modulation of catalytic activity. Several metalloproteins behave as chelators for specific metals with no known catalytic reactions. Such heavy metal binding sites exist in metallothioneins and in various protein elements of bacterial heavy metal mechanisms and have been exploited for specific detection through affinity events. Nevertheless and as previously mentioned, bacterial resistance mechanisms can also be linked to catalytic pathways. For instance, c5rtochromes c3 and hydrogenases from sulfate and sulfur reducing bacteria [284,285] are well suited for bioremediation purposes because they can reduce various metals such as U(V) and Cr(VI) [286,287]. Cytochrome c3 has been reported to catalyse Cr(VI) and U(VI) reduction in Desulfovibrio vulgaris [288,289], suggesting... 

Heterodimeric [NiFe]-hydrogenase crystal structures have been reported for four closely related sulfate-reducing bacteria from Desulfovibrio sp. D. gi-gas [40,41], D. vulgaris (Miyazaki) [42 - 44], D. fructosovorans [45,46] and D. desulfuricans [47]. Overall, the structures are very similar being roughly... 

The microenviromnent of AOT/isooctane reverse micelles was considered as a biomimetic system for the solubilization of flavodoxin, aldehyde oxidoreductase, and a membrane-associated hydrogenase, all isolated from the sulfate reducing bacteria Desulfovibrio gigas [153,154], The main perspective of this research group was the encapsulation of aU the three microbial enzymes mentioned above in reverse micelles and the observation of the electron-transfer chain under the water restricted conditions. 

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