Sugar phosphates, preparation

The vicinal diol groups of deoxy sugar phosphates can be cleaved by periodate in the usual conditions without affecting the stability of the phosphate group, so that this reaction can be used for preparative purposes in certain cases. Thus, if 3-deoxy glucose 6-phosphate (23) is... 

Several 1 -phosphates of deoxyfluoro sugars were prepared, and their acid-catalyzed hydrolysis was studied. 2-Deoxy-2-fluoro- (580), 3-deoxy-3-fluoro- (582), 4-deoxy-4-fluoro- (583), and 6-deoxy-6-fluoro-a-D-gluco-pyranosyl phosphates (584) were prepared by treatment of the corresponding per-( -acetylated )9-D-glucopyranoses with phosphoric acid [the p anomer (581) of 580 was prepared by a different method]. The first and second ionization constants (pA a, and pA a2) of these compounds were determined potentiometrically, as well as by the F-n.m.r. chemical shifts at a series of pH values, and then the rate constants of hydrolysis for neutral (B) and monoanion (C) were decided. The first-order rate-constants (k) for 580-584 and a-D-glucopyranosyl phosphate (in Af HCIO4,25 °) were 0.068, 0.175, 0.480, 0.270, 1.12, and 4.10 (all as x lOVs), respectively. The rate... 

The following diphosphates and triphosphates can be prepared by reaction of nucleotide azolides with nucleotides, sugar phosphates, phosphopeptides, or aminohexyl phosphates, a) NAD-analogues and P1, / -dinucleoside-S -diphosphates 801... 

Of course, it is now established that the glycolysis of glucose to carbon dioxide and ethanol occurs by way of a complex pathway involving 10 different enzymes acting on a variety of sugar phosphate intermediates. The extracellular enzyme preparation that Fischer used was termed invertin, the origin of the term for the enzyme we now know as invertase. He termed the intracellular enzyme yeast-glucase (31) and this enzyme is of the type we now refer to as an amylase. 

Additional problems of anomeric phosphorylation are the control of anomeric selectivity and the increased lability of the products. The preparation of anomeric sugar phosphates can be accomplished via two distinct methods. The first commences with a free anomeric hydroxyl and can be considered as a standard phosphorylation. The second approach... 

Sugar phosphates, mainly the hexose mono- and di-phosphates, play an important part in alcoholic fermentation. The calcium salts of these esters have been prepared. 

Figure 3 Conversion of ribose S-phosphate to sugar phosphate products by rat liver enzyme preparation (RLEP) (40). Details of the incubation, protein content and methods used are described in (42). For measurement of the max. catalytic capacity of RLEP see (43). Reprinted from International Journal of Biochemistry, 19. Williams John. F., Arora Krishan K. and Longnecker John P. The pentose pathway A random harvest, 69 Pages, 1987, with permission from Elsevier, http //www.sciencedirectcom/science/journal/l 3S7272S...

The inhibition of telomerase is dependent on the ability of the modified ODN to form a stable duplex with the RNA target and not on the nature of the sugar phosphate backbone. The most active ODNs were in the sub-nM to pM range of concentrations. a-ODNs containing a mixture of phosphodiester and cationic phosphoramidate (N-dimethylaminopropylphosphoramidate) linkages were prepared and evaluated as TFOs." Such ODNs were found to be superior third strands in triplexes compared to 3-phosphodiester ODNs over a 5.5 - 7.0 pH range. 

Polyterpenoids.—Needles of Juniperus communis contain a range of polyprenols up to ca. Choj major component being the C95 alcohol (137). The series of syntheses of polyisoprenyl sugar phosphates has been extended to dolichyl /8-d-mannopyranosyl phosphate (138). A simple procedure for the preparation of... 

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