Sugars chromatography

Palu, M. and Samuelson, O., Influence of aromatic compounds in sugar chromatography on anion-exchange resins, Talanta, 24, 264, 1977. 

Figure 3. Decomposition of sprucewood holocellulose by xylanase 2 + mannanase, monitored by quantitative sugar chromatography. Ordinate degradation in percent of dry holocellulose based on the amount of reducing sugars in the reaction solution. ( --total degradation products,...

Mopper, K. and Gindler, E.M., 1973. A new non-corrosive dye reagent for automatic sugar chromatography. Anal. Biochem., 56 440—442. 

HPLC High pressure liquid chromatography. Hudson s isorotadon rule For a pair of sugars... 

Sucrose quantitation has also been performed by colorimetric methods. However, in recent years, automated enzymatic analyzers and instmmental methods (eg, ion chromatography and hplc) have become increasingly popular, as they provide greater sensitivity and accuracy. Near infrared (nir) spectroscopy is currendy under evaluation as a tool for sucrose quantitation in sugar mills and food processing operations. 

The diphenylmaleimide is prepared from the anhydride, 33-87 % yield, and cleaved by hydrazinolysis, 65-75% yield. It is stable to acid (HBr, AcOH, 48 h) and to mercuric cyanide. It is colored and easily located during chromatography, and has been prepared to protect steroidal amines and amino sugars. " ... 

Heikkila H. (1983) Separating Sugars and Amino Acids with Chromatography, Chem. Eng., January 24, 50-52. 

The only recorded example using this method in the sugar series is the chlorination of l,2 3,4-di-0-isopropylidene-D-galactopyranose (73) which affords in addition to the expected 6-chloro-6-deoxy derivative 74a, a 5,6-unsaturated derivative 75 as well. These products were separated by silica gel column chromatography no yields were given. 

The purpose of the experiment is to illustrate the application of derivatisation in the analysis of sugar and related substances by gas-liquid chromatography. The silylation method described is an almost universal derivatisation procedure for carbohydrate analysis by GC.79... 

To an ice-cold solution of the aldehydo sugar in CH,OH (ca. 25 mL per g) are added 3 equiv of nitromethane (or nitrocthane), followed by 2.9 equiv of sodium methoxide. After stirring for 2 h 45 min, the medium is neutralized by the addition of acidic Dowex 50, then filtered. Removal of the solvent by distillation and purification by chromatography on silica gel (Ei,0/hcxane) or on aluminum oxide (Et20) furnishes the product yield 85-99%. 

Laboratory colonies of worker ants were fed sugar-water containing l-C14-acetate, 2-C14-acetate, or in a carefully controlled simultaneous feeding, 1-C14- or 2-C14-mevalonate. After an appropriate period, the ants were frozen and extracted with methylene chloride and the terpene aldehydes (citronellal and citral) isolated by thin-layer chromatography. These were then converted into their dini-... 

In contrast, another group35 found that extracts of E. coli contained a mixture of pentulose phosphates at a concentration near 0.3 nmol per mg of the dry weight of cells. The sugars were estimated by gas chromatography-mass spectrometry after treatment of the extract with phosphatase followed by silylation, or borohydride reduction and acetylation. Furthermore, a partially purified preparation from these extracts catalyzed the synthesis of 1-deoxypentulose... 

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