Sugars paper chromatography

Shortly afterwards, Westphal, Liideritz, and their coworkers using the newly developed method of paper chromatography, found a new class of sugars in lipopolysaccharides (LPS) from Gram-negative bacteria, and identified them as 3,6-dideoxyhexoses. This work is summarized in Ref. 4. These discoveries initiated more-systematic investigations of hydrolyzates from bacterial polysaccharides, and a number of new monosaccharides were completely or partially identified. This development has been summarized by Ashwell and Hickman. ... 

The identities of the individual sugars were established by acid hydrolysis of a portion of the sample followed by paper chromatography and comparison with authentic samples. It was interesting to note that one of the sugars was the rarely encountered (S-D-allose that has only been found in a few saponins. Regrettably, only scant details were reported on the acquisition conditions used for the 1,1-ADEQUATE spectrum. 

A comparative analytical study, by means of paper chromatography, has confirmed the conclusion that, in the condensation of aldoses with ethyl acetoacetate, the yield of the furan derivatives increases as the length of the sugar chain is decreased.17... 

Siddapa GS and Bhatia BS. 1954. The identification of sugars in fruits by paper chromatography. Indian J Hort 11 104. 

Rivas, M. M. Sugars and polyols in olive seed. I. Identification by paper chromatography and gas-liquid-chro- OE071 matography. Grasas Aceites (Seville)... 

A similar, if somewhat sketchier, study of the photolysis of d-UpU (16b, R = H) has been carried out,73 and comparison with the photolysis of UpU is interesting. The close similarity of d-UpU and UpU, differing only in the sugar moiety, and there only slightly, would lead to the expectation that the photolyses should be very similar in outcome. The differences are, however, appreciable. d-UpU forms only two dimers, and both the single and double hydrate are separable by paper chromatography, leading to a reaction scheme as shown in Chart 4. 

Nevertheless, hydrolysis of a gum arabic solution in an acid medium and analysis of the sugars by paper chromatography reveals the presence of the following sugars ... 

Fifty years after the discovery of apiose, a review of the literature1 showed that it was still a curiosity, and had been found only in the parsley plant. Apart from its structure, and those of the flavonoids from which it was isolated (primarily, apiin), little else was known about apiose. In the next two decades, this branched-chain sugar was shown to have very widespread occurrence in the plant kingdom, being identified, in most of the plants investigated,4-6 by paper chromatography. 

Incubation of a cell-free extract of L. minor in the presence of NAD+ and UDP-D-[U-14C]glucuronic acid generated two compounds, each containing a different radioactive C5 sugar.96 The presence of apiose after hydrolysis was diagnosed by (a) paper chromatography, (b) specific complex-formation with benzeneboronic acid, (c) electrophoretic mobility, and (d) formation of its isopropylidene acetal. Treatment with ammonia at 0° showed that the compound was formed by a nonenzymic cyclization.96 Treatment with phosphoric diesterase plus phosphatase further supported an earlier report of the existence of a D-apiosyl cyclic phosphate.95... 

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