Substrate induced conformation change

Lopez-Corcuera, B., Nunez, E., Martinez-Maza, R Geerlings, A., and Aragon, C. (2001) Substrate-induced conformational changes of extracellular loop 1 in the glycine transporter GLYT2. J. Biol. Chem. 276,43463-13470. 

Loo, T.W., Bartlett, M.C. and Clarke, D.M. (2003) Substrate-induced conformational changes in the transmembrane segments of human P-glycoprotein. Journal of Biological Chemistry, 278, 13603-13606. 

ISOTOPE TRAPPING STICKY SUBSTRATES Substrate-induced conformational change, INDUCED FIT MODEL SUBSTRATE INHIBITION ABORTIVE COMPLEX FORMATION LACTATE DEHYDROGENASE LEE-WILSON EQUATION... 

Bernstein BE, Michels PAM, Hoi WGJ. Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation. Nature 1997 385 275-278. 

Rittinger K, Divita G, Goody R. Human immunodeficiency virus reverse transcriptase substrate-induced conformational changes and the mechanism of inhibition by nonnucleoside inhibitors. Proc Natl Acad Sci USA 1995 92 8046-8049. 

Second step fast. Reaction 4.47 could involve a substrate-induced conformational change in the enzyme, where ES is just a different conformational state, or, alternatively, it could involve the accumulation of an intermediate on the pathway. The following reaction illustrates the displacement of an equilibrium between two conformational states of an enzyme caused by the binding of a substrate to one form only ... 

Chang YT, Loew GH. Molecular dynamics simulations of P450 BM3-exami-nation of substrate-induced conformational change. J Biomol Struct Dyn 1999 16 1189-1203. 

The 35 GHz Mn(II) spectrum of this enzyme is severely broadened by ATP and other nucleotide analogs, permitting a characterization of substrate-induced conformational changes at the active site of the (Na+ + K+)-ATPase. In particular we have used the diminution of the Mn(II) EPR spectrum upon titration with B,7 -bidentate CrATP to determine a Mn(II)-Cr(III) distance in the ATPase-Mn-CrATP complex. On the other hand, gadolinium ion, Gd(III), is an effective probe of the Ca(ll) sites of the Ca2+-ATPase of sarcoplasmic reticulum. Thus Li+ NMR and water proton... 

A physical technique for the study of conformation based on measuring changes in heat capacity of a molecule under various conditions. See Zecchinon, L., Oriol, A., Netzel, U. et al.. Stability domains, substrate-induced conformational changes, and hinge-bending motions in a psychro-philic phosphoglycerate kinase. A microcalorimetric study, J. Biol. Chem. 280, 41307-41314, 2005. 

Qasba PK, Ramakrishnan R, Boeggeman E. Substrate-induced conformational changes in glycosyltransferases. Trends Biochem Sci. 2005 30 53-62. 

As an alternative to the substrate-induced conformational change that occurs after substrate binding, the mechanism according to Scheme XIII has been considered, with a conformational change preceding binding. 

This corresponds to a decrease of only 4 ergs/cm. One possible explanation for these observations is that HSA undergoes more extensive substrate induced conformational changes than the more rigid IgG and fibrinogen molecules. 

Figure 16.46, Yeast PGK-ATF complex. Numbers indicate positions of sing tiyplt lians used as intrinsic fluoiescent pfobes lo delect substrate-induced conformational changes. Three helices situated In the hinge region, V. X]]l, and Xi V, are riiowninblaclc. Reprinted, wiApennissioii. ftom Ref. 121, Copyright O 1996, Cambridge University Press.

Y., Yasuoka, N., and Toraya, T. (2002) Substrate-induced conformational change of a coenzyme B12-dependent enzyme crystal structure of the substrate-free form of diol dehydratase. Biochemistry, 41 (42), 12607-12617. 

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