Subject direction

B. 5-Hexynal. To a solution of 5.60 g. (0.050 mole) of 2,3-epoxycyclohexanone in 120 ml. of benzene in a 500-ml. round-bottomed flask is added 10.82 g. (0.051 mole) of trans-1 -amino-2,3 diphenylaziri-dine.2 Initially, after brief swirling at room temperature, the reaction mixture is a colorless, homogeneous solution however it rapidly turns yellow and cloudy due to separation of water. After 2 hours the benzene and water are removed as an azeotrope under reduced pressure on a rotary evaporator with the bath maintained at approximately 30°. The resulting crude mixture of diastereomeric hydrazones weighs 15.4 g. (Note 7) and is subjected directly to the fragmentation reaction (Note 8). 

Not surprisingly, the cobalt-complexed ether products obtained by the Nicholas reaction can be subjected directly to the Pauson-Khand sequence (Scheme 7).244... 

Gauthier et al. <2005JOC5938> elaborated a synthetic route to 148, which is an important biologically active compound. These authors found that 147 can be subjected directly to cross-coupling process with the appropriate boronic acid, and there is no need for the halogenation of 147 to an intermediate for this cross-coupling. The product 148 was obtained in kilogram quantities in almost quantitative yield. 

The reaction effluent of the rhodium process is subjected to flash distillation under mild conditions in order to maintain the activity of the sensitive catalyst and to contain the costly metal in a limited section of the plant. The effluent of the nickel process is subjected directly to distillation of the products with considerable savings. 

Proteins blotted on PVDF can be subjected directly to sequence analysis. Specialized protocols for this purpose are referred to, for example, by JuNGBLUT (1997). 

After completion the yellow solution is concentrated in a vacuum (10 2 torr) to one quarter of its original volume and subjected directly to column chromatography on Sephadex G-15. Yield 856 mg (89%, based on Co) canary-yellow powder. 

PATENTABILITY. The qualifications for obtaining a patent on an invention of chemical process. These arc (1) the invention must not nave been published in any country or in public use in the U.S., in either case for more than 1 year before the date of filing the application (2) it must not have been known in the IJ.S. before date of invention by the applicant (3) it must not be obvious to an expert in the art (4) it must be useful for a purpose not immoral and not injurious to the public welfare (5) it must fall within the five statutory classes on which patents may be granted, i.e., (a) composition of matter, (b) process of manufacture or treatment, (c) machine, (d) design (ornamental appearance), or (e) a plant produced asexually. Special regulations relate to atomic eneigy developments and subjects directly affecting national security (Robert Calvert). Note In 1980, the Supreme Court m al landmark decision upheld the patentability of synthetic bacteria created by recombinant DNA techniques. 

If the ligand protein is fluorescently labeled, resuspend the cells in 10 pL PBS and subject directly to FACS. If the ligand protein is biotinylated (protocol 2), resuspend the cells in 10 pL PBS containing streptavidin, R-phycoerythrin conjugate (1 10 dilution in PBS). Incubate on ice again for 10 min. 

A dry test tube containing catalyst (1.4 mg, 2 pmol, 2 mol%) and the imine (44.4 mg, 0.15 mmol, 1.5 equiv) was purged with nitrogen and then charged with toluene (1 mL). The resulting solution was treated with neat tert-butyl diazoacetate (14.2 mg, 0.1 mmol, 1 equiv) at r.t. After stirring for 24 h at ambient temperature, the mixture was subjected directly to FC on silica gel (hexanes ethyl acetate, 10 1 to 3 1) to give the product (32.9 mg, 75 pmol, 75% yield, 95% ee) as an oil. 

An individual atom or ion in a dielectric is not subjected directly to an applied field but to a local field which has a very different value. Insight into this rather complex matter can be gained from the following analysis of an ellipsoidal solid located in an applied external field Ea, as shown in Fig. 2.28. The ellipsoid is chosen since it allows the depolarizing field Edp arising from the polarization charges on the external surfaces of the ellipsoid to be calculated exactly. The internal macroscopic field Em is the resultant of Ea and Edp, i.e. Ea — Edp. 

The possibility of preliminary isolation of substances under analysis in the form of hydrazones is frequently utilized in the determination of ketosteroids in complex mixtures such as biological materials. In this form they are even sometimes subjected directly to GC analysis. Charransol et al. [378] determined androstanediol and testosterone in urine. After hydrolysis the sample was extracted with methylene chloride, evaporated and treated with Girard T reagent. Free hydroxysteroids were extracted with diethyl ether and... 

The procedure shown here describes the preparation of a-silyl ketones from aldehydes and acyl chlorides. The a-silyl ketones undergo Cram addition of various nucleophiles to produce diastereoselectively p-hydroxysilanes. These compounds are then subjected directly to elimination in situ under basic or acidic conditions to produce the corresponding alkenes. 

GC-MS can be used to analyse mescaline in casework samples [11]. In this example, dried, powdered material was soaked in chloroform for 20 min, the mixture centrifuged, and the supernatant recovered and evaporated to dryness. The material was reconstituted in 0.5 ml of methanol and then subjected directly to GC-MS analysis, using the operating conditions given in Table 7.3. 

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