Subcutaneous inoculation

The DNA forms stable complexes with doxorubicin (Adriamycin, ADR) and daunorubicin (DNR). Doxorubicin and DNR, although structurally similar, show distinctly different properties ADR is more toxic and active than DNR in the treatment of various human solid tumors the apparent binding affinity of ADR to DNA is about 1.8 times higher than that of DNR to DNA. Trouet et al. [229] found the ADR-DNA complex to be more active than ADR, DNR, or DNR-DNA in subcutaneously inoculated leukemic mice, whereas the DNR-DNA complex showed the highest... 

Key words Subcutaneous inoculation, Streptococcus pyogenes, skin and soft tissue infection. 

In assessing the efficacy of in vitro versus in vivo production of reagent antibody, consideration must be given both to the relative concentrations of antibody and to the volumes obtainable. Subcutaneous inoculation of cells in a mouse yields approximately 1 ml of serum 2 weeks later the yield of ascitic fluid harvested 7-14 days after intraperitoneal injection is between 5 and 15 ml. As the concentration of antibody produced in this way is at least 100-fold greater than in tissue culture, 10 ml of ascitic fluid from one mouse would be equivalent to at least 1 liter of tissue culture fluid. In this context, the recent description of hybridomas produced by fusion of a rat myeloma line with rat spleen cells is likely to be of considerable practical significance because of the larger volume of serum obtainable following inoculation of hybridomas in these rodents. ... 

Subcutaneous inoculations are convenient but the response is slower than by the intramuscular route. It is recommended for boosters since it decreases the chances of an anaphylactic shock. The needle (bevel up) is inserted into the fold of the skin, lifted between thumb and forefinger, until the tip of the needle feels free. After the injection the needle track should be pinched to prevent loss of inoculated... 

Figure 1. Infiltration of F4/ 80+ cells (monocyte/ macrophage) into the m uscle of Swiss outbred mice at day 8 post Ross River virus infection (which corresponded with the onset of clinical disease). Mice were subcutaneously inoculated at day 14-15 days of age with 10 plaque-forming units per mouse oftheT48 strain of Ross River virus. A) Sham-inoculated B) day 4 post Ross River virus infection C) day 8 post Ross River virus infection (200x magnification). Reprinted with permission from Lidbury BA et al. Macrophage-induced muscle pathology results in morbidity and mortality for Ross River virus-infected mice. J Infect Dis 2000 181 27-34. 2000 University of Chicago Press.

Apffel CA, Peters JH. Rejection of lethal tumor after subcutaneous inoculation a phenomenon of antigenic expression J Natl Cancer Inst. 1967 39 1129-39. 

Zaucha, G.M., Pitt, L.M., Estep, J., et al, 1998. The pathology of experimental anthrax in rabbits exposed by inhalation and subcutaneous inoculation. Arch. Pathol. Lab. Med. 122, 982-992. 

---