Styragel column calibration curve

Tables 11.1 through 11.4 give useful ranges for the various Styragel and Ultrahydrogel packings. It should be pointed out that Styragel packings with the designation E at the end of the name are mixed-bed columns with an extended range of the calibration curve. To view the full range of each calibration curve, go to the sections that discuss the individual column types.

FIGURE I 1.6 Calibration curves of Waters Styragel HR columns. (Courtesy of Waters Corp.)... 

Molecular weights were determined using a Waters high-pressure GPC instrument (Model 6000 A pymp, a series of five p-Styragel columns (10s, 10s, 10, 103, 500 A), Differential Refractometer 2401 and UV Absorbance Detector Model (440) and a calibration curve made by well fractionated polyisobutylene standards. 

Figure 1. Calibration curve for four -styragel columns CPS in TCB at 135 °C).

The molecular mass characteristics of PP and copolymers were determined at 135°C in o-dichlorobenzene on a Waters 150-C gel chromatograph equipped with a linear HT-p-styragel column. The average molecular mass was calculated from the universal calibration curve plotted relative to PS standards. 

The Styragel 1000 column (1.0 x 42 cm) was prepared from resin swollen with benzene-methanol (90 10 v/v). Polystyrene standards were used for molecular weight calibration, and an exclusion limit of 28,000 was determined. The Sephadex-excluded asphaltene fraction was chromatographed on the Styragel 1000 column and a continuous elution curve was obtained from molecular weight 22,000 to 1000. 

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