Streptomyces carbophilus

Pravastatin 4 Pravastatin Anti-cholesterol Hydroxylation Streptomyces carbophilus Single-stage fermentation [5]... 

Matsuoka T, Miyakoshi S et al (1989) Purification and characterization of cytochrome P450 from Streptomyces carbophilus. Eur J Biochem 184 707-713... 

T Matsuoka, S Miyakoshi, K Tanzawa, K Nakahara, M Hosobuchi, N Serizawa. Purification and characterization of cytochrome P-450sca from Streptomyces car-bophilus. ML-236B (compactin) induces a cytochrome-P450sca in Streptomyces carbophilus that hydroxylates ML-236 B to pravastatin sodium (CS-514), a tissue-selective inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Eur J Bio-chem 184 707-713, 1989. 

Milbemycin derivatives can be stereoselectively hydroxylated at the allylic 13p-position by a variety of microorganisms including Streptomyces violascens (ATCC 31560), Streptomyces carbophilus (FERM BP-1145) or Streptomyces diastatochromogenes (ATCC 31561), and in preparatively useful yields. ... 

Pravastatin (CS-514) (19) has been produced by microbial transformation of mevastatin (compactin, ML-236B) [41,42]. Cytochrome P-450sca from Streptomyces carbophilus was described in detail as being responsible for this conversion [43]. It differs from lovastatin in that it contains a P-hydroxyl group at C-6 position of the hexahydronaphthalene ring. This modification makes it more hydrophilic than lovastatin. In... 

Ito, S., T. Hata, 1. Watanabe, T. Matsuoka, and N. Serizawa (1997). Crystal structure of cytochrome P450sca from Streptomyces carbophilus involved in production of pravastatin, a cholesterol lowering drug. FASEBJ. 11, 36. 

For the preparation of pravastatin (Mevalotin , Sankyo), at first mevastatin is obtained from fermentation of Penicillium citrinum its regio- and stereo-selective enzymatic hydroxylation can be achieved with a number of different microbial strains Mucor hiemalis, Penicillium coccineus, Rhizoctonia solani or Nocar-dia. Streptomyces carbophilus is used today to produce mevastatin under optimised conditions a productivity of around 5 g/1 or even higher can be obtained. [392]... 

Class II Bacteria, microsome, plants, fungi NAD(P)H CPR- P450 Streptomyces carbophilus CYP105A3 (P450sca) [165] Microsomal P450s [166]... 

Watanabe I, Nara F, Serizawa N (1995) Cloning, characterization and expression of the gene encoding cytochrome P-450sca-2 from Streptomyces carbophilus involved in production of pravastatin, a specific HMG-CoA reductase inhibitor. Gene 163 81-85... 

Watanabe I, Serizawa N (1998) Molecular approaches for production of pravastatin, a HMG-CoA reductase inhibitor transcriptional regulation of the cytochrome p450sca gene from Streptomyces carbophilus by ML-236B sodium salt and phenobarbi-tal. Gene 210 109-116... 

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