Streptavidin-fluorescein isothiocyanate

Biotin and biotin analogs Infant formula Protein precipitation using concentrated hydrochloric acid neutralization with 6 M NaOH lipid extraction with n-hexane Precolumn Microsorb C18 (15 X 4.6 mm, 5 jam Rainin). Analytical Microsorb C18 (250 X 4.6 mm, 5 /zm Rainin). Isocratic 100 mM phosphate buffer, pH 7.0 + methanol (80 + 20, v/v). 0.4 ml/min. Postcolumn reaction system UV absorbance at 220 nm followed by streptavidin-fluorescein isothiocyanate (2.0 mg/L) knitted open tubular reaction system (10.0 m x 0.5-mm ID) at a flow rate External standardization. 184 Linear range = 0.08-1.00 fjM biotin. LoD = 0.02 /zM or 97 pg biotin at SNR = 3. Repeatability CV 3.5% for biotin in infant formula. 

To qualitatively investigate the effect of altering the amount of magainin I grafted onto the copolymer brush surface, the brushes were functionalised with a biotin-tagged derivative (biotinyl-MAG-Cys) and then incubated with streptavidin-fluorescein isothiocyanate (streptavidin-FITC) the surface-immobilised streptavidin-FITC... 

Nano-sized iron oxide pjartides (Fe304) were coated with a lipid layer and then functionalized with biotin for further protein ligands. These particles were then used for binding streptavidin-fluorescein isothiocyanate generating a conjugate used to investigate their magnetic and fluorescent activity into cells (Beckera et al., 2007). 

Fluorescein isothiocyanate (FITC) is the most common fluorescent label used to modify proteins and other biomolecules (Chapter 8, section 1.1). The isothiocyanate group reacts with amines in protein molecules to form a stable thiourea linkage (Fig. 365). Avidin or streptavidin may be tagged with this reagent to yield highly fluorescent... 

The traditional radioimmunoassay technique could be utilized with rare earth phosphate NPs. Lin et al. reported the LuP04-apoferritin coreshell NPs synthesized in apoferritin templates. The NPs were further modified with biotin and exhibited affinity for streptavidin-modifed magnetic beads or streptavidin-modifed fluorescein isothiocyanate. Radioactive Lu was used to label the NPs (Wu et al., 2008a). 

NPH HCl) with l-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC HCl) as a coupling agent [593,594], The advantage is that with hydrazines it is possible to carry out the derivatization in milder conditions. An efficient postcolumn derivatization is obtained using avidin or streptavidin. In fact, these two proteins react in a very specific way with biotin, therefore they are bound with a fluorescent marker, such as fluorescein 5-isothiocyanate (FITC) to obtain fluorescent derivatives. Some authors [595] report the use of MS or MS/MS for biotin detection, but this method seems to be less sensitive than FLD. 

The effects of CVS on the numbers of T-cell subsets were examined on day 9 at the time of tumor i.v. rechallenging, Fig. (6)-Protocol B. Cells were stained with fluorescein 5-isothiocyanate (FITC)-, phycoerythrin (PE)-conjugated and/or biotinylated mAb and were additionally stained with RED-613-streptavidin for flow-cytometric three-color analysis. 

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