Stratum corneum tape stripping

Stratum Corneum Tape Stripping Relationship with Dry Skin and Moisturizers... 

Chapter 35 Stratum Corneum Tape Stripping Relationship with Dry Skin and Moisturizers 457 Frank Dreher and Howard I. Maibach... 

Pellett, M. A., M. S. Roberts, and J. Hadgraft. 1997. Supersaturated solutions evaluated with an in vitro stratum corneum tape stripping technique. Int.. Pharm. 151 91-98. 

Bashir, S.J., Chew, A.L., Anigbogu, A., Dreher, E, and Maibach, H.I., 2001, Physical and physiological effects of stratum corneum tape-stripping. Skin Res. TechnoL, 1,40-48. 

Tregear, R.T., and P. Dirnhuber. 1962. The mass of keratin removed from the stratum corneum by stripping with adhesive tape. J Invest Dermatol 38 375. 

Drug levels within the stratum corneum can be assessed by sampling single corneocyte layers with adhesive film. The drug is then extracted from the tape-strips and quantified by a suitable analytical method. Usually, scintillation counting (for radioactive compounds) or high performance liquid... 

Determination of the stratum corneum depth by Tape-Strip Number... 

For untreated skin, repeated stripping may remove a constant amount of stratum corneum, independent of the number of strips already performed [125], The momentary depth may thus be calculated by dividing the mean thickness of the overall stratum corneum by the total number of tapes necessary to remove it completely and multiplying with the number of strips already performed. 

J.-C. Tsai, M. J. Cappel, N. D. Weiner, G. L. Flynn, and J. Ferry. Solvent effects on the harvesting of stratum corneum from hairless mouse skin through adhesive tape stripping in vitro. Int. J. Pharm. 68 127-133 (1991). 

F. Dreher, B. S. Modjtahedi, S. P. Modjtahedi, and H. I. Maibach. Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates. Skin Res. Technol. 11 97-101 (2005). 

Weerheim A, Ponec M (2001) Determination of stratum corneum lipid profile by tape-stripping in combination with high-performance thin-layer chromatography. Arch Dermatol Res 293 191-199. 

Stripped refers to removal of part of the stratum corneum by adhesive tape application. 

Jacobi, U., et al. 2005. Estimation of the relative stratum corneum amount removed by tape stripping. Skin Res Technol 11 91. 

Loffler, H., F. Dreher, and H.I. Maibach. 2004. Stratum corneum adhesive tape stripping Influence of anatomical site, application pressure, duration and removal. Br J Dermatol 151 746. 

Feingold and his coworkers demonstrated an important role of nuclear hormone receptor on epidermal differentiation and stratum corneum barrier formation. Activation ofPPARa Peroxisome proliferator-activated receptor a by farnesol also stimulated the differentiation of epidermal keratinocytes.42 Cornified envelope formation, involcrin, and transglutaminase protein, and mRNA levels were also increased by the activation of PPARo . Interestingly, the inflammatory response was also inhibited by the treatment.43 They also showed that topical application of PPARo activators accelerated the barrier recovery after tape stripping or acetone treatment and prevented the epidermal hyperplasia induced by repeated barrier disruption.42 Regulation of the nuclear hormone receptor would open a new possibility for improvement of the cutaneous barrier. 

Yang, L., Man, M.Q., Taljebini, M., Elias, P.M., and Feingold, K.R. (1995) Topical stratum corneum lipids accelerate barrier repair tape stripping, solvent treatment and some but not all types of detergent treatment. Br. J. Dermatol. 133 679-685. 

Redoules, D. et al., Characterisation and assay of five enzymatic activities in the stratum corneum using tape-strippings, Skin Pharmacol. Appl. Skin Physiol., 12, 182, 1999. 

Evaluation of the occlusive properties of different pastes in vivo these were evaluated in vivo on tape-stripped skin exactly as described in Reference 10. Briefly, the stratum corneum of the forearm of healthy volunteers was tape stripped until the transepidermal water loss (TEWL) attained values between 40 and 50 g/m2/h. After a rest period of 1 h, 2.5 mg/cm2 of the test products were carefully applied on the stripped sites using a gloved finger, and the TEWL was measured at different times until 120 min after application. Percent changes relative to a nontreated control site were calculated over time. Positive control was white petrolatum. 

Lanolin appears to penetrate into the stratum corneum, but remains in the more superficial layers.38 39 Using the tape stripping technique, Clark demonstrated the penetration of anhydrous lanolin (2 mg/cm2) applied to the flexor aspect of the inner forearm.40 Almost all of the applied lanolin was recovered and most was removed in the first 15 strippings. Although the bulk of lanolin may remain in the superficial layers, electron-dense lead linoleate and lead oleate topically applied in lanolin were observed by transmission electron microscopy to be localized in intracellular spaces as far down as the stratum granulosum 40... 

The outermost skin layer, the stratum corneum (SC), consists of corneocytes embedded in lipid layers and represents the main barrier for skin penetration of xenobiotics. Its thickness in healthy adults may vary from 5 to 20 /um, except in the palm and sole where it is much thicker. SC can be removed sequentially by repeated application of appropriate adhesive tapes.1 This technique, commonly known as SC tape stripping, is a relatively noninvasive method to investigate the structure, properties, and functions of SC in vivo2 and is the most frequently used for such purposes. Other techniques to remove SC are skin surface biopsy using cyanoacrylate strips and skin scraping. 

Van der Molen, R.G., Spies, F., Van t Noordende, J.M., Boelsma, E., Mommaas, A.M., and Koerten, H.K., Tape stripping of human stratum corneum yields cell layers that originate from various depths because of furrows in the skin. Arch. Dermatol. Res. 289, 1997, 514—518. 

Marttin, E., Neelissen-Subnel, M.T.A., De Haan, F.H.N., and Bodde, H.E., A critical comparison of methods to quantify stratum corneum removed by tape stripping. Skin Pharmacol. 9, 1996, 69-77. 

Dreher, F., Arens, A., Hostynek, J.J., Mudumba, S., Ademola, J., and Maibach, H.I., Colorimetric method for quantifying human stratum corneum removed by adhesive-tape-stripping. Acta Dermatol. Venerol. (Stockh.) 78, 1998, 186-189. 

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