Steroid hormone-activated gene networks

Pratt-Kaye, S., Bellard, M., Dretzen, G., Bellard, F. and Chambon, P. (1986) EMBO 

11Department of Endocrinology, Academic Hospital Utrecht and bDepartment of Biochemistry ll, Erasmus University Rotterdam, The Netherlands 

Steroid hormones achieve their effects on target tissues through intracellular receptor proteins. According to recent views, oestrogen and progestin receptors are localized in the nuclear compartment of the cells, whereas glucocorticoid receptors may reside in both the cytoplasm and the nucleus. Determination of the intracellular localization of androgen receptors awaits the development of (monoclonal) antibodies which will enable immunohistochemical studies. The molecular aspects of the mechanism of action of steroid hormones will be covered in other chapters [1-3] in this volume. The present chapter deals with the characterization, assay and purification of steroid receptors. 

Correspondence to M.A. Blankenstein, Department of Endocrinology, Academic Hospital Utrecht, P.O. Box 16250, 3500 CG Utrecht, The Netherlands. 

---