States inclusion

The unequivocal proof is furnished by the crystal inclusion behavior of simple 2-hydroxy-3-naphthalenecarboxylic acid 25a46 , and its 1-chloro derivative 25b37 since both allow the formation of a crystalline adduct ( clathratocomplex 19)) with dimethylformamide with the expected 1 1 stoichiometric ratio37. Thus, the salicylic acid function (2-hydroxycarboxylic acid group) is shown to be an excellent sensor, or a good complementary site for the dimethylformamide molecule in solid state inclusion. 

Toda and Akai49 reported that compound 48 reacted with the stable solid state inclusion compound of chiral host 46 and meso-ketone 47, providing alkene 49 in 57% ee. 

Host and guest are terms covering all kinds of intermolecular associations from the well-defined supermolecules to loosely packed solid state inclusion compounds. 

A major class of insoluble proteins are recombinant proteins expressed (usually in Escherichia coli) as inclusion bodies. These are dense aggregates found inside cells that consist mainly of a desired recombinant product, but in a nonnative state. Inclusion bodies may form for a variety of reasons, such as insolubility of the product at the concentrations being produced, inability to fold correctly in the bacterial environment, or inability to form correct, or any, disulfide bonds in the reducing intracellular environment. Their purification is simple, since the inclusion bodies can be separated by differential centrifugation from other cellular constituents, giving almost pure product the problem is that the protein is not in a native state, and is insoluble. Some methods for obtaining an active product from inclusion bodies are described in Coligan et al. (2001). 

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