Stain lissamine green

Lissamine green is a vital stain that stains degenerate cells, dead cells, and mucus in much the same way as rose bengal. It is also widely used in the food industry as a colorant. 

In contact lens wear it has become apparent that conjunctival staining in general is related to symptoms of irritation and that lissamine green in particular may be more specific compared with fluorescein for those with symptoms. 

Instillation of lissamine green B into the conjunctival sac appears to cause no ocular irritation, and no other adverse effects have been reported. Clinical experience suggests the staining effect of lissamine green to be longer lasting than that of rose bengal. 

Chodosh J, Dix RD, Howell RC, et al. Staining characteristics and antiviral activity of suUbrhodamine B and lissamine green B. Invest OphthahnolVis Sci 1994 35 1046-1058. 

Manning F, Wehrly SR, Foulks GN. Patient tolerance and ocular surface staining characteristics of lissamine green versus rose bengal. Ophthalmology 1995 102 1953-1957. 

Norn MS. Lissamine green. Vital staining of cornea and conjunc-tiva.Acta Ophthalmol 1973 51 483-491. 

NaFl and rose bengal stains can be instiUed simultaneously. Several grading scales for quantifying ocular staining with NaFl, rose bengal, and lissamine green have been... 

G16. Gorringe, J. A. L., Lissamine Green as a protein stain in paper electrophoresis. Clin. Chim. Acta 2, 353-371 (1957). 

Gorringe (G4) stains with lissamine green (BDH) using a solution containing 0.30 g dye in a mixture of 15.0 ml acetic acid and 100.0 ml distilled water. This stain can also be applied to films which have been previously stained with Sudan black, thus achieving a simultaneous coloration of proteins and lipids (W4). 

Rose bengal and lissamine green, which also are available as a 1% solution and as saturated paper strips, stain devitalized tissue on the cornea and conjunctiva. 

Lissamine green staining reagent (0.3 g dissolved in 15% (v/v) acetic acid). 

Staining of serum strips. After the required time turn off the current and remove the strips. Immerse them in the Lissamine green staining reagent for 5 min. Wash the strips three times in 1 % (v/v) acetic acid to remove the excess dye and blot dry. Dry the strips in a stream of warm air. Record your results both quantitatively and qualitatively. The dry strips may be rendered glass clear by immersion in Whitmore oil if required. 

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