Stachyose acetate

In the original isolation of stachyose described8 by Planta and Schulze, the minced tubers of Stachys tuberifera were pressed and extracted with water. The juice, after clarification with lead acetate and mercuric nitrate, was treated with hydrogen sulfide. After removing the metal sulfides and neutralizing the filtrate with ammonia, the... 

Thanks to the very great tendency of stachyose to crystallize from sirups treated with glacial acetic acid (the suggestion of Dr. E. D. Walter), it is possible in this way to by-pass preliminary separation of the stachyose from other sugars present. On the other hand, the precipitation is not so complete but that a certain amount of stachyose remains in the acetic-acid mother liquor, from which it can be recovered only with some difficulty. 

Tri- and tetrasaccharides such as raffinose and stachyose may be separated with the chromatographic conditions given in Fig. 3-105. When adding sodium acetate to the... 

Tri- and tetrasaccharides such as raffinose and stachyose may be separated under the chromatographic conditions given in Figure 3.208. As with phosphorylated monosaccharides, shorter analysis times are obtained when adding sodium acetate to the eluent. The same holds for the analysis of tri-and tetrasaccharides of the kestose group, which also function as artificial sweeteners. 

Structure. Cryoscopic molecular weight values indicate that verbascose is a pentasaccharide 169). From methylation studies, Murakami concluded that verbascose was a D-galactosyl-substituted stachyose (at C-6 of the terminal D-galactose unit of stachyose). Hot dilute acetic acid removes the D-fructose unit from verbascose to give a tetrasaccharide verbascotetraose (m.p. 240°C. [a] +191.4 (water)). This tetrose has been partially hydrolyzed, with removal of D-glucose, to give the crystalline trisaccharide galactotriose 164)-... 

Free Sugars and Simple Derivatives Thereof.— -D-Galactopyranose and a-L-fucopyranose, a-o-altropyranose penta-acetate, and stachyose (the first tetrasaccharide to be so examined). ... 

Fig. 1. Electrophoresis of sugars (A) sucrose, raffinose, and fructose (B) sucrose, raffinose, melibiose, and glucose (C) sucrose, raffiinose, stachyose, and fructose 40 v./em., 8-12 milliamperes, 1.5 hours, 1.5 X 22.5 inch strip, Whatman No. 1, 0.05 M borate buffer solution, pH — 9.2 (B) sprayed with benzidine + acetic acid, (A), (0), and (D) with 1-naphthol 4- phosphoric acid (D) kestose, sucrose, raffinose, and stachyose same conditions except for 3 hour run and origin line in center of strip instead of 9 inches from cathode end 5-20 ig. of each sugar applied.

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