Spots pale pink

Signs Appears as small dark sunken spots that spread rapidly over the berry. Spots may have a pale pink crust on their surface. Berry may become mummified prior to full development. Otherwise, the berry ripens and the bean can become infected. Crop... 

Suspend the purified salt in 850 ml of water in a stoppered bottle, add 25 ml of pure pyridine and shake the mixture for 4-5 hours. Remove the insoluble pyridine rhodanilate by filtration and wash it with 100 ml of cold water (2). Combine the pale pink filtrate and washings, and add glacial acetic acid dropwise until the formation of a small pink precipitate is complete. Filter, evaporate the almost colourless filtrate to dryness (rotary evaporator) and suspend the residue in absolute ethanol and re-evaporate twice. Dry the resulting faintly pink crude proline in a vacuum desiccator over silica gel the yield is about 18 g. Recrystallise from the minimum volume of absolute ethanol to obtain 11 g (7.3% based on gelatin) of L-proline, m.p. 218-219 °C (decomp.), [oc]d8 —85.6° (c3.0 in H20). Check the purity of the product by t.l.c. on silica gel using the solvent system butan-l-ol-acetic acid-water, 4 1 1 Rf 0.26 (yellow spot with ninhydrin). 

Many standards describing paper to be used for conservation purposes will specify that the papers should be free of alum and lignin. Several spot tests can be used to detect these and other materials found in paper one of each will be described. One test for alum uses a solution of a chemical called alu-minon. The pale pink solution is applied to the surface of the paper and allowed to dry. A pale pink spot is produced when no aluminium salts are present a darker pink or red is seen when such salts are present. Lignin can be detected using a freshly prepared solution of phloroglucinol in hydrochloric acid. A drop of the colourless solution is applied to the paper and a red colouration indicates the presence of lignin. There are several tests for rosin. 

Phenols 4-Amino antipyrine Red, orange, or pink spots on pale background... 

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