SPOT-technique

Multiple peptide synthesis with the SPOT-technique 

SPOT-synthesis is a facile and very flexible technique for the simultaneous parallel solid phase chemical synthesis. Series of compounds or compound 

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Scheme 7.26 Library generation on a cellulose membrane employing the SPOT technique.

Refs 1) R.T. Eckenrode, The Spotting Technique Effect of Ambient Illumination, Spot... 

Marian, The Spotting Technique The Question of Spot vs Smoke and Spot Duration , Memo for Record, Project TS44020, FrankfordArs (Nov 1964) 6) J.A. Carrazza et al, Development of... 

The Spot Technique Synthesis and Screening of Peptide Macroarrays on Cellulose Membranes... 

Frank, R., Kiess, M., Lahmann, H., Behn, C., and Gausepohl, H. (1995) Combinatorial synthesis on membrane supports by the SPOT technique. In Peptides 1994 Proceedings of the 23rd European Peptide Symposium, ed. H.L.S. Maia, pp. 479-480. Leiden ESCOM. 

The scanning laser spot technique allows the lateral resolution of the photoelec-trochemical properties of the semiconductor and permits the visualization of the defects (Furtak and Parkinson, 1980). Figure 10.30 shows the results of sweeping and laser spot over a step in InSe. A step is a recombination center and the recombination of holes and electrons increases. 

The resolution obtained with the laser spot technique is far exceeded by scanning tunneling microscopy where, in some cases, atomic resolution in electrochemical cases has been reached (Szklarczyk and Velev, 1989). The first successful studies of semiconductors in air (Fig. 10.31) and in an electrochemical situation (Fig. 10.32) were made onp-Si 111 (Gonzalez-Martin, 1990). It was found that the electrochemical formation of SiOx and SiOH induces surface states at 0.25 V above the valence band at the surface (Fig. 10.33). 

Adler, F., Turk, G., Frank, R., et al. (2000) A new array format for the automated parallel combinatorial synthesis by the SPOT-technique. In Innovation and Perspectives in Solid Phase Synthesis (Epton, R., ed.), Mayflower Worldwide, Kings-winford, UK, pp. 221-222. 

Fig. 32.14 Double-spotting technique (a) compounds A and B with close flf values (b) figure 8 of double spot shows that A and B are different (c) single spot for double spot shows that A and B could be the same.

However, there are other manipulations performed for chromatography that aflFect the precision and accuracy of the final result. As mentioned previously, the spotting technique variation was minimized by the use of an automatic spotter (35) which spots sample and standard under similar parameters, and the size of the initial spots are adjusted so that the Einstein-Smoluchowsky eflFect is minimized 42), allowing a range of concentrations from the minimum detectable to 20 /mg. 

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