Sponge bioassays

A number of tetramic acid derivatives acylated with a liposaccharide unit have recently been discovered in marine sponges. The first example was uncovered in the sponge Ancorina sp using a bioassay to detect inhibition of blastulation in starfish embryos. The structure of the compound (27), named ancorinoside A, was determined by spectroscopic techniques... 

By the bioassay-guided fractionation of the crude extract of the marine sponge Axinella weltneri, a new triterpene sodwanone S 138 with an uncommon cyclohexanooxepane system was isolated <2005JNP1284>. 

Thompson, J. E., Walker, R. P., and Faulkner, D. J., Screening and bioassays for biologically-active substances from forty marine sponge species from San Diego, California, USA, Mar. Biol., 88, 11, 1985. 

Bioassay guided fractionation of the methanol extract of an Indonesian sample of I. hasta resulted in the isolation of a total of eight bastadins representing each of the three structural motifs found in the series [128,129]. Bastadin 6 (114) was isolated as the major bioactive constituent of the sponge and inhibited the VEGF- and... 

Bioassay-guided fractionation of an aqueous extract from a Philippine Islands collection of the soft coral Lohophytum spp. yielded cembranoid diterpenes, Fig. (3), which exhibited moderate HIV-inhibitory activity in a cell-based in vitro anti-HIV assay [44], while new isomalabaricane triterpenes. Fig. (4), have been isolated from the sponge Stelletta spp. [45]. Other anti-HIV diterpenes also included the dolabellane diterpenes isolated from the Brazilian brown algae Dictyota pfaffi [46] and Dictyota menstrualis [47]. To investigate the effect of these diterpenes in the reverse transcription of the viral genomic RNA, the recombinant HIV-1 RT was assayed in vitro in the presence of each compound. All compounds inhibited the RNA-dependent DNA-polymerase activity of HIV-1 RT and consequently virus replication. 

Extracts of Cribrochalina sp, a marine sponge, displayed antifungal activity. Bioassay guided fractionation led to the isolation of cribrochalinamine /V-oxides A and B (21a,b). Structures of these compounds were determined using HRFABMS, and one- and two-dimensional JH and 13CNMR[118],... 

Plakinamine B (330) was isolated from a marine sponge Plakina sp., using bioassay-guided fractionation [695]. Its structure was determined using and 13 q NMR and UV. The hydrochloride salt of 330 inhibited the growth of Staphylococcus aureus and Candida albicans [695],... 

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