Sphingomyelin identification

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Kerwin, J.L., Tuininga, A.R., Ericsson, L.H. 1994. Identification of molecular species of glycerophospholipids and sphingomyelin using electrospray mass spectrometry. J. Lipid Res., 35, 1102-1114. 

Kim, M. Y., Linardic, C, Obeid, L., and Hannun, Y., 1991, Identification of sphingomyelin turnover as an effector mechanism for the action of tumor necrosis factor alpha and gamma- interferon. Specific role in cell differentiation. J. Biol. Chem. 266 484-489... 

Huitema K, van den Dikkenberg J, Brouwers JF, Holthuis JC. Identification of a family of animal sphingomyelin synthases. Embo / 23(2004) 33 4. 

Elmendorf, H. G. and Haidar, K. (1993) Identification and localization of ERD2 in the malaria parasite Plasmodium falciparum separation from sites of sphingomyelin synthesis and implications for organization of the Golgi. EMBO J. 12 AKri-AllTi. 

We describe the utility of intermediate-pressure MALDI and tandem mass spectrometry (MS/MS and MS ) for the characterization and imaging of phospholipids in brain tissue sections. The use of both MS/MS spectra and MS/MS images allows for identification of isobaric compounds. The structural characterization of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines, and sphingomyelins directly fi om tissue sections is described. 

The methods presented here describe the ability to characterize phosphatidylcholines (PCs), sphingomyelins (SPMs), phos-phatidylserines (PSs), and phosphatidylethanolamines (PEs) in the positive ion mode from rat brain tissue sections using imaging tandem MS and the creation of compound-specific images from full-scan MS and MS, while using MS for the final identification. The use of MS images for the separation of isobaric ions coupled with the ability to perform MS provides the means to identify isobaric species present in the tissue section. 

Houjou, T., Yamatani, K., Nakanishi, H., Imagawa, M., Shimizu, T. and Taguchi, R. (2004) Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3. Rapid Commun. Mass Spectrom. 18,3123-3130. 

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