Spectrophotometric Methods of Analysis

EDTA forms colored complexes with a variety of metal ions that may serve as the basis for a quantitative spectrophotometric method of analysis. The molar absorptivities of the EDTA complexes of Cu +, Co +, and Ni + at three wavelengths are summarized in the following table (all values of e are in cm )... 

The method described next is used with all of the spectrophotometric methods of analysis described earlier that is, standard solutions are prepared and analyzed, and standard curves created and statistical analyses carried out. The final step of relating the results back to the original sample and field is also essential and the same. 

Introduction of photoelectric cells led to the replacement of the Duboscq colorimeter and so to quantitative spectrophotometric methods of analysis which met biochemical requirements. This introduction of spectrophotometry as a routine procedure was one of the earliest technological advances underpinning the elucidation of biochemical pathways between 1930-1960. Micromanometric methods also became available about the same time, and offered a means to measure cell respiration. Manometry was developed in Warburg s laboratory in Berlin and was one of the main techniques used by H.A. Krebs in his studies on the citric acid and urea cycles (Chapters 5 and 6). 

Elemental Analysis Titrimetric Analysis Electrochemical Analysis Spectrophotometric Methods of Analysis... 

The results for exposed samples of Illinois No. 6 coal indicate that 0.11 0.06 wt% elemental sulfur is present. All the methods except the bacteriological method, which appears to underestimate the sulfur content, provide reasonably consistent results. While not highly precise, the four physicochemical methods provide accurate information, and suggest that 1 to 5% of total sulfur in the exposed coals from this Basin is in the elemental form. It is pertinent to note that the spectrophotometric method of analysis that has been developed by Buchanan and Chaven does not involve gas chromatography and is especially suitable for the analysis of the concentration of small amounts of sulfur in extracts (8). 

Spectrophotometric method of analysis of rare earth elements. 61... 

Spectrophotometric methods of analysis of rare earths are given in Tables 1.34 and 1.35. 

Chapters 1-4 provide the characteristics of the separation and preconcentration methods solvent extraction, flotation, coprecipitation with collectors, volatilization, ion exchange etc. These chapters deal also with the fundamentals of spectrophotometry, spectrophotometric methods of analysis, and most important chromogenic reagents. Chapters 5-57 have been devoted to individual elements or groups of related elements. 

If the absorbance increases by unity, the transmittance drops by a factor of ten. Equation (24.10) is an expression of the Beer-Lambert law, often called simply Beer s law. Beer s law is the basic equation for the various colorimetric and spectrophotometric methods of analysis. If Beer s law holds, then the absorbance is given by... 

Spectrophotometric Methods of Analysis Thin Layer Chromatographic Methods of Analysis Gas-Liquid Chromatographic Methods of Analysis High Performance Liquid Chromatographic Methods of Analysis... 

The radiation absorption and transmission characteristics of polymers can be used to identify polymer types. The basic principle of spectrophotometric methods of analysis is to provide an energy source (such as infrared radiation, IR) to which a sample is exposed, and to convert to some measure a response that is characteristic of the moiecuies. This response is, most often, absorption of a fraction of the incident tight. 

Spectrophotometric method of analysis using barbituric acid-pyridine in the distillate... 

Haematoxylin, haematein (the coloring principles of logwood spectrophotometric method of analysis and spectra of pure compounds behavior of aqueous solutions with varying pH pK values... 

Table 26.2 Solution Spectrophotometric Methods of Analysis for Water...

---