Specificity of antibodies against

BH Wainer, FW Fitch, J Fried, RM Rothberg. Immunochemical studies of opioids Specificities of antibodies against codeine and hydromorphone. Clin Immunol Im-munopathol 3 155, 1974. 

For example. If the antibodies used In an Immunoassay for aflatoxin (AF) are 1001 cross-reactive with aflatoxin B1 (AFBl), but only 33X with AFG1, the presence of AFG1 In the sample would result In a lower "apparent" total AF level by the Immunoassay method (16). Thus, It Is very Important to know the specificity of the antibody to be used In the Immunoassays. The specificity of antibody against various mycotoxins has been documented In a number of reviews (1-6, 17). For the purpose of discussion, the diversity of antibody specificity for aflatoxins (AFs) and triochothecenes (TCTCs) are presented here. 

Specificity of antibodies against aflatoxin and related metabolites The relative cross-reactivity of different types of antibodies against aflatoxin with some selected AF analogs are Illustrated In Fig. 3. The Importance of the site of conjugation to the antibody specificity Is well demonstrated from these... 

The observed specificity of antibodies against thousands of different antigens indicates that there are cells waiting to be stimulated into multiplication and production of one or another of thousands of different... 

Boldicke, T., Kindt, S., Maywald, F., Fitzlaff, G., Bocher, M., Frank, R., and Collins, J. (1988) Production of specific monoclonal antibodies against the active sites of human pancreatic secretory trypsin inhibitor variants by in vitro immunization with synthetic peptides. Eur. J. Biochem. 175, 259-264. 

Schlessinger 1990). These phosphorylation events stand at the beginning of specific signaling cascades that ultimately lead to transcription initiation and cell mitosis or differentiation. Because of the complexity of the proteome, it is at this stage most reasonable to think about protein chips that have a defined number of antibodies against known disease markers. 

G., Garegg, P.J., Svensson, S., and Wallin, N.-H. (1979). Immuno-chemistry of Salmonella O-antigens. Specificity and cross-reactivity of factor 09 serum and of antibodies against tyvelose a-(l—3)-mannose coupled to bovine serum albumin. Int. Archs Allergy appl. Immun. 58, 11-19... 

We propose that this advanced technology has clear advantages over conventional methods for selective generation of novel monoclonal antibodies. It may also allow efficient production of stereo-specific monoclonal antibodies against native structure antigens with application of antigen-expressing myeloma cells. 

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