Human pancreatic secretory trypsin

Boldicke, T., Kindt, S., Maywald, F., Fitzlaff, G., Bocher, M., Frank, R., and Collins, J. (1988) Production of specific monoclonal antibodies against the active sites of human pancreatic secretory trypsin inhibitor variants by in vitro immunization with synthetic peptides. Eur. J. Biochem. 175, 259-264. 

Kanamori, T., et al. 1988. Expression and excretion of human pancreatic secretory trypsin inhibitor in lipoprotein-deletion mutant of Escherichia coli. Gene 66 295. 

Human pancreatic secretory trypsin inhibitor (hPSTI) can be potentially assayed as an indicator of necrotic complications in AP (Ol). This protein is an inhibitor of trypsinogen, which is produced in acinar cells in the quantity of approximately 2% of the potential content of trypsin in pancreas. Trypsin binds with its inhibitor hPSTI, then with AMG, and only this complex, trypsin-o 2-macroglobulin, is eliminated from plasma (B10). Pezzili (P3) suggests that early attempts to determine the severity of the AP process based on the measurement of hPSTI within 24 hr from the first sensations of pain show a sensitivity of 79%, whereas an increase in CRP concentration has a sensitivity of 29% only (Table 3). 

P3. Pezzilli, R., Human pancreatic secretory trypsin inhibitor in assessment of severity of acute pancreatitis A comparison with C-reactive protein. J. Clin. Gastroenterol. 19,112-117 (1994). 

CONSENSUS SEQUENCES FOR HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (PSTI) VARIANTS, SELECTED BY PHAGE DISPLAY IN THE pSKAN8 VECTOR, WHICH INHIBIT EITHER BOVINE CHYMOTRYPSIN (CHY) OR HUMAN LEUKOCYTE ELASTASE (HLE) WITH SUBNANOMOLAR K, VALUES (REF. 72 AND P. ROTTGEN AND J. COLLINS, UNPUBLISHED DATA)... 

There appears to be sequence homology between the pineapple stem bromelain inhibitors and some of the small molecular weight inhibitors from the legumlnosae (91) Human inter-a-trypsin inhibitor contains two domains with great similarity to the domains of the Kunitz-type inhibitors (44 92-94)> The ovoinhibitors from Japanese quail and chicken egg whites contain six tandem domains which are homologous to the Kazal pancreatic secretory inhibitor and to the ovomucoids (69) ... 

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